首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human Interleukin 5,IL-5 ELISA KIT

  • 中文名： 人白介素5(IL-5)ELISA Kit
货号: IPD-E04636h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人白介素5(IL-5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL5含量。IL5 是重要的治疗靶点。它主要由 Th2 细胞等产生，可促进嗜酸性粒细胞的生长、分化、存活及活化。在哮喘等疾病中，嗜酸性粒细胞过度活化参与炎症反应。针对 IL5 开展研究，旨在阻断其信号通路，抑制嗜酸性粒细胞相关炎症，为相关疾病治疗提供新方向。试剂盒检测范围为3.9 pg/ml-250 pg/ml，灵敏度为0.97 pg/ml。适用于科研实验中IL - 5水平的动态监测。在基础医学研究领域，可用于评估疾病模型动物血清中细胞因子表达谱、分析炎症微环境中组织匀浆的免疫调节机制，或体外药物干预实验的疗效验证，为免疫调节机制研究及潜在治疗靶点探索提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    B-cell differentiation factor I ELISA Kit; Colony stimulating factor ELISA Kit; EDF ELISA Kit; Eosinophil differentiation factor ELISA Kit; IL-5 ELISA Kit; IL5 ELISA Kit; IL5_HUMAN ELISA Kit; Interleukin 5 (colony stimulating factor; eosinophil) ELISA Kit; Interleukin 5 ELISA Kit; Interleukin-5 ELISA Kit; T Cell Replacing Factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
  • 缩写：
    IL5
  • Uniprot No.：
    P05113
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.9 pg/ml-250 pg/ml
  • 灵敏度：
    0.97 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    82-96


    1:2

    Average %

    92


    Range %

    90-105


    1:4

    Average %

    95


    Range %

    92-103


    1:8

    Average %

    90


    Range %

    86-98


  • 回收率：

    The recovery of human IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    85

    80-92


    EDTA plasma (n=4)

    87

    82-94


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    250

    2.973

    2.834

    2.904

    2.754


    125

    2.276

    2.167

    2.222

    2.072


    62.5

    1.248

    1.228

    1.238

    1.089


    31.25

    0.635

    0.629

    0.632

    0.483


    15.6

    0.363

    0.371

    0.367

    0.218


    7.8

    0.269

    0.253

    0.261

    0.112


    3.9

    0.217

    0.214

    0.216

    0.066


    0

    0.144

    0.155

    0.149




  • 货期：
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×