| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GPX4; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase;;GPX4 |
| WB Predicted band size | Calculated MW: 22 kDa ; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GPX4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于GPX4抗体的3篇示例文献(内容为模拟概括，仅供参考)：
1. **文献名称**：*GPX4 regulates ferroptosis and cell death in cancer*
**作者**：Yang WS, Stockwell BR
**摘要**：该研究通过Western blot和免疫组化使用GPX4抗体，证实GPX4通过抑制脂质过氧化调控铁死亡，其失活可诱导癌细胞死亡，为癌症治疗提供新靶点。
2. **文献名称**：*Inactivation of GPX4 triggers ferroptosis in vivo*
**作者**：Conrad M, Friedmann Angeli JP
**摘要**：利用基因敲除模型和GPX4抗体检测，发现GPX4缺失会导致小鼠器官中铁死亡激活，揭示了GPX4在维持细胞氧化稳态中的关键作用。
3. **文献名称**：*Characterization of GPX4 antibody specificity in neurodegenerative models*
**作者**：Schneider KS, et al.
**摘要**：研究比较了多种市售GPX4抗体的特异性，验证了其在脑组织样本中的适用性，为神经退行性疾病中GPX4蛋白表达分析提供了实验依据。
(注：以上为示例性内容，实际文献需通过学术数据库检索确认。)
The glutathione peroxidase 4 (GPX4) antibody is a critical tool in studying the role of GPX4. a selenium-containing antioxidant enzyme essential for cellular redox homeostasis. GPX4 uniquely reduces lipid hydroperoxides within membranes, thereby preventing ferroptosis—a form of regulated cell death driven by iron-dependent lipid peroxidation. This enzyme is pivotal in mitigating oxidative damage, particularly in tissues with high metabolic activity, such as the liver, brain, and kidneys. Dysregulation of GPX4 is linked to pathologies including cancer, neurodegenerative diseases, and ischemia-reperfusion injury.
GPX4 antibodies are widely used in research to detect protein expression and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate GPX4's involvement in ferroptosis modulation, its interaction with pathways like the glutathione and mevalonate systems, and its role in therapeutic resistance. For instance, cancer studies exploit GPX4 antibodies to explore tumor survival mechanisms, as GPX4 inhibition can sensitize cells to ferroptosis inducers. Similarly, in neurodegenerative research, these antibodies aid in understanding how GPX4 depletion exacerbates lipid peroxidation in conditions like Alzheimer’s disease.
The development of specific, high-affinity GPX4 antibodies remains vital for advancing mechanistic insights and therapeutic strategies targeting ferroptosis-related diseases.
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