| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DAZ homolog; DAZ like autosomal; DAZH; DAZL; DAZL1; DAZLA; Deleted in azoospermia like 1; Germline specific RNA binding protein; SPGY like autosomal; SPGYLA; Tpx2;;DAZ homolog |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human DAZ homolog |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DAZL抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *The DAZL family of genes are essential for germ cell development*
**作者**: Ruggiu, M., et al.
**摘要内容**: 该研究通过基因敲除实验证实DAZL蛋白在小鼠精子发生中的关键作用，并利用特异性抗体检测到其在减数分裂前生殖细胞中的表达，表明DAZL对生殖细胞分化至关重要。
2. **文献名称**: *A novel antibody marker for human germ cells*
**作者**: Kee, K., et al.
**摘要内容**: 文章开发了一种高特异性人源DAZL抗体，通过免疫组化和流式细胞术验证其在人类睾丸组织及多能干细胞分化模型中标记原始生殖细胞的应用，为生殖医学研究提供工具。
3. **文献名称**: *DAZL expression in human oocytes and preimplantation embryos*
**作者**: Clark, A.T., et al.
**摘要内容**: 研究利用DAZL抗体分析人类卵母细胞和早期胚胎中蛋白的时空表达模式，发现其在卵子成熟和胚胎发育早期的调控作用，提示DAZL缺陷可能与女性不孕相关。
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以上文献涵盖DAZL抗体的开发验证、生殖细胞标记及临床相关性研究，可作为相关领域的基础参考。如需扩展，可进一步检索近年抗体应用或单细胞测序结合DAZL标记的研究。
The DAZL (Deleted in Azoospermia-Like) antibody is a critical tool for studying germ cell development and reproductive biology. DAZL, a member of the DAZ gene family, encodes an RNA-binding protein essential for gametogenesis in both males and females. It is highly conserved across vertebrates and plays a pivotal role in regulating meiosis, germ cell maturation, and fertility by binding to target mRNAs to modulate their translation. DAZL is predominantly expressed in germ cells, making it a key marker for tracking germline development in models ranging from humans to zebrafish.
DAZL antibodies are widely used in research to detect and localize DAZL protein expression in tissues, embryos, or in vitro cell cultures. They enable investigations into germ cell specification, infertility mechanisms, and the differentiation of pluripotent stem cells into germline lineages. Mutations or deficiencies in DAZL are linked to human infertility, particularly non-obstructive azoospermia in males and premature ovarian insufficiency in females. Studies using DAZL antibodies have also advanced reproductive technologies, such as in vitro gametogenesis, by identifying molecular pathways critical for germ cell survival and function.
These antibodies are typically validated for applications like Western blotting, immunohistochemistry, and immunofluorescence, supporting both basic research and clinical diagnostics in reproductive medicine.
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