首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素3(IL-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL3含量。IL3，即白细胞介素-3，是一种由激活的T细胞和其他细胞产生的细胞因子，主要在造血过程中发挥作用，调控粒细胞和单核细胞-巨噬细胞的产生、分化和功能。其研究机制涉及IL3如何通过激活细胞内信号通路，如Ras-Raf-ERK和JAK/STAT，来促进造血细胞的生长和分化。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域中对IL-3表达水平的研究，例如在造血系统疾病模型、感染性疾病动物实验或免疫相关病理机制探讨中，通过检测血液或组织样本中的IL-3浓度变化，揭示其在炎症反应、细胞分化及免疫调控中的作用规律。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il3 ELISA Kit; Il-3Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
缩写：IL3
Uniprot No.：P04823
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-113 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of rat IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.4362.2762.3562.2141001.9752.0141.9951.853501.4351.3721.4041.262250.8680.9130.8910.74912.50.5530.5190.5360.3946.250.3870.4070.3970.2553.120.2850.2760.2810.13900.1440.1400.142 | ||||||
货期：3-5 working days
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