| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DHFR; DHFRP1; Dihydrofolate reductase; DYR;;Dihydrofolate reductase |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Dihydrofolate reductase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4条关于DHFR(二氢叶酸还原酶)抗体的参考文献摘要：
1. **"Production and characterization of a monoclonal antibody specific for human dihydrofolate reductase"**
- 作者：C. R. Wagner 等
- 摘要：该文献描述了针对人类DHFR的单克隆抗体的制备与特性分析，通过免疫印迹和免疫沉淀实验验证了抗体的高特异性，并证明其可用于检测内源性及重组DHFR的表达水平。
2. **"Application of anti-DHFR antibodies in studying drug resistance in cancer cells"**
- 作者：S. P. Cole 等
- 摘要：研究利用DHFR抗体分析甲氨蝶呤耐药癌细胞中DHFR的过表达现象，揭示了基因扩增与蛋白表达水平的关系，为抗肿瘤药物研究提供了实验依据。
3. **"Cross-species reactivity of anti-DHFR antibodies and their use in developmental biology"**
- 作者：T. L. DeVito 等
- 摘要：探讨了DHFR抗体在小鼠、斑马鱼等模式生物中的交叉反应性，验证了其在胚胎发育过程中叶酸代谢相关研究的适用性。
4. **"CRISPR/Cas9-mediated tagging of DHFR for antibody validation in live-cell imaging"**
- 作者：K. M. Hyodo 等
- 摘要：通过CRISPR/Cas9技术对DHFR进行内源性荧光标记，结合抗体共定位实验验证了商用DHFR抗体的可靠性，为活细胞成像提供了质量控制方法。
注：以上文献名为示例概括，实际文献需通过PubMed或学术数据库检索确认具体信息。
The dihydrofolate reductase (DHFR) antibody is a critical tool in biomedical research for studying DHFR, an essential enzyme involved in nucleotide synthesis and cellular metabolism. DHFR catalyzes the reduction of dihydrofolate to tetrahydrofolate, a cofactor required for thymidine and purine synthesis, making it vital for DNA replication and cell proliferation. Due to its role in cell growth, DHFR is a target for chemotherapeutic agents like methotrexate, which inhibit its activity to suppress rapidly dividing cancer cells.
DHFR antibodies are widely used to detect and quantify DHFR protein expression in cells and tissues, aiding in research on cancer biology, drug resistance mechanisms, and folate metabolism. They are also employed in studies investigating DHFR overexpression, a common resistance mechanism in cancer cells exposed to antifolate therapies. Additionally, these antibodies support applications in molecular biology, such as validating CRISPR-edited DHFR knockouts or monitoring DHFR-tagged fusion proteins in transfection experiments.
Available as monoclonal or polyclonal variants, DHFR antibodies are optimized for techniques like Western blotting, immunohistochemistry, and flow cytometry. Researchers rely on their specificity to distinguish endogenous DHFR from exogenous forms in gene expression studies. The antibody’s utility extends to infectious disease research, as microbial DHFR enzymes (e.g., in *Plasmodium* or bacteria) are drug targets, and species-specific antibodies help evaluate pathogen responses to antifolate treatments. Overall, DHFR antibodies serve as indispensable tools for understanding cellular proliferation, drug development, and metabolic pathways.
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