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Human Interleukin 3,IL-3 ELISA KIT

  • 中文名： 人白介素3(IL-3)ELISA Kit
货号: IPD-E04631h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素3(IL-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的IL3含量。IL3是重要靶点。它是一种细胞因子，主要由活化T细胞等产生。在机体造血调控、免疫调节等方面起关键作用。研究围绕其信号通路展开，旨在了解其在疾病发生发展机制，为开发靶向IL3的治疗药物，如单抗等提供依据。试剂盒检测范围为62.5 pg/ml-4000 pg/ml.，灵敏度为15.6 pg/ml.。适用于基础医学研究中细胞因子网络调控机制探索、疾病动物模型中的IL-3水平动态监测、药物干预效果评价等科研场景。为免疫学、肿瘤学、血液病学等领域的体外实验研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Colony stimulating factor multiple ELISA Kit; Hematopoietic growth factor ELISA Kit; IL 3 ELISA Kit; IL-3 ELISA Kit; IL3 ELISA Kit; IL3_HUMAN ELISA Kit; Interleukin 3 (colony stimulating factor; multiple) ELISA Kit; Interleukin 3 ELISA Kit; Interleukin-3 ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; MGC79398 ELISA Kit; MGC79399 ELISA Kit; Multi CSF ELISA Kit; Multilineage colony stimulating factor ELISA Kit; Multipotential colony stimulating factor ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; OTTHUMP00000065963 ELISA Kit; P cell stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
  • 缩写：
    IL3
  • Uniprot No.：
    P08700
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    62.5 pg/ml-4000 pg/ml.
  • 灵敏度：
    15.6 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    93


    Range %

    86-102


    1:2

    Average %

    94


    Range %

    91-105


    1:4

    Average %

    96


    Range %

    90-102


    1:8

    Average %

    90


    Range %

    86-97


  • 回收率：

    The recovery of human IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    85-94


    EDTA plasma (n=4)

    91

    87-97


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    4000

    2.510

    2.634

    2.572

    2.422


    2000

    1.922

    1.867

    1.894

    1.745


    1000

    1.325

    1.228

    1.276

    1.127


    500

    0.874

    0.829

    0.852

    0.702


    250

    0.489

    0.481

    0.485

    0.336


    125

    0.343

    0.353

    0.348

    0.198


    62.5

    0.245

    0.234

    0.240

    0.090


    0

    0.154

    0.145

    0.150




  • 货期：
    3-5 working days

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