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Human Interleukin 29(IL-29)ELISA Kit

  • 中文名: 人白介素29(IL-29)ELISA Kit
货号: IPD-E14290h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人白介素29(IL-29)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的IL29含量。IL29,又称白细胞介素-29或干扰素λ1,是一种近年发现的细胞因子,属于干扰素λ家族。它通过结合特定的异二聚体受体复合物来发挥生物学作用,与Ⅰ型干扰素共享相同的Jak-STAT信号传导途径,具有抗病毒、抗增殖、抗肿瘤和免疫调节等生物学活性。试剂盒检测范围为62.5 pg/ml-4000 pg/ml.,灵敏度为15.6 pg/ml.。适用于细胞实验、动物模型或临床样本中IL-29的定量分析,可支持免疫调节机制研究、抗病毒药物开发评价以及炎症相关疾病的基础科研,适用于分子生物学、免疫学等领域的蛋白表达水平研究。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    IFNL1 ELISA Kit; IL29 ELISA Kit; ZCYTO21Interferon lambda-1 ELISA Kit; IFN-lambda-1 ELISA Kit; Cytokine Zcyto21 ELISA Kit; Interleukin-29 ELISA Kit; IL-29 ELISA Kit
  • 缩写:
    IL29
  • Uniprot No.:
    Q8IU54
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates.
  • 检测范围:
    62.5 pg/ml-4000 pg/ml.
  • 灵敏度:
    15.6 pg/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-29 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    96


    Range %

    90-102


    1:2

    Average %

    97


    Range %

    91-105


    1:4

    Average %

    97


    Range %

    94-103


    1:8

    Average %

    94


    Range %

    87-98


  • 回收率:

    The recovery of human IL-29 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    89

    82-96


    EDTA plasma (n=4)

    97

    92-100


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    2.128

    Average

    Corrected


    4000

    2.128

    2.028

    2.078

    1.974


    2000

    1.427

    1.327

    1.377

    1.273


    1000

    0.778

    0.748

    0.763

    0.659


    500

    0.394

    0.374

    0.384

    0.280


    250

    0.248

    0.238

    0.243

    0.139


    125

    0.200

    0.194

    0.197

    0.093


    62.5

    0.154

    0.153

    0.154

    0.050


    0

    0.105

    0.103

    0.104




  • 货期:
    3-5 working days

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