| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 21 kDa TBP-like protein; Second TBP of unique DNA protein; STUD; TATA box-binding protein-related factor 2; TBPL1; TLF; TLP; TLP21;;TBPL1 |
| WB Predicted band size | Calculated MW: 21 kDa ; Observed MW: 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TBPL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是假设的关于TBPL1抗体的参考文献示例(内容基于类似研究主题虚构，供参考)：
1. **文献名称**：*TBPL1 regulates oocyte maturation through transcriptional control in mice*
**作者**：Zhang Y, et al.
**摘要**：研究利用TBPL1特异性抗体，通过免疫组化和Western blot分析，揭示TBPL1在小鼠卵母细胞成熟过程中调控特定基因转录的功能，证实其缺失导致减数分裂异常。
2. **文献名称**：*TBPL1 as a novel biomarker in hepatocellular carcinoma progression*
**作者**：Wang L, et al.
**摘要**：通过TBPL1抗体检测肝癌组织中TBPL1蛋白表达水平，发现其高表达与患者预后不良相关，并证明TBPL1通过调控Wnt通路促进肿瘤侵袭。
3. **文献名称**：*Functional characterization of TBPL1 in zebrafish embryogenesis*
**作者**：Chen R, et al.
**摘要**：利用TBPL1抗体进行斑马鱼胚胎免疫荧光染色和ChIP实验，发现TBPL1在早期胚胎发育中调控中胚层分化相关基因的表达。
4. **文献名称**：*TBPL1 interacts with RNA polymerase II during cellular stress response*
**作者**：Kim S, et al.
**摘要**：通过TBPL1抗体的免疫共沉淀(Co-IP)实验，证明其在应激条件下与RNA聚合酶II结合，参与DNA损伤修复相关基因的转录调控。
注：以上文献为示例性质，实际文献需通过数据库(如PubMed)检索确认。
**Background of TBPL1 Antibody**
TBPL1 (TATA-box-binding protein-like 1), also known as TRF2 or TRP, is a member of the TBP family of transcription factors. Unlike the canonical TBP protein, which is essential for RNA polymerase II-mediated transcription initiation, TBPL1 plays specialized roles in germ cell development, embryogenesis, and oocyte-specific gene regulation. It functions as a component of the transcriptional machinery, binding to TATA-box-like DNA sequences and interacting with other transcription factors to regulate target gene expression.
TBPL1 antibodies are critical tools for studying its expression, localization, and molecular interactions. These antibodies enable the detection of TBPL1 in various experimental applications, including Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP). Research has highlighted TBPL1's importance in fertility and early development, as knockout models in mice result in embryonic lethality or sterility. Additionally, TBPL1 dysregulation has been implicated in certain cancers and reproductive disorders, underscoring its biomedical relevance.
The development and validation of TBPL1 antibodies require careful consideration of specificity, given the structural homology among TBP family members. High-quality antibodies facilitate investigations into TBPL1's unique transcriptional mechanisms and its role in cellular differentiation, stress responses, and disease pathways.
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