| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CANP2, mCANP, CANPL2, CANPml |
| WB Predicted band size | 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAPN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GCN2(EIF2AK4)抗体的3篇代表性文献的简要概括(注：文献信息为示例性总结，具体引用需核对原文)：
1. **文献名称**：*GCN2 sustains mTORC1 suppression upon amino acid deprivation by associating with CASTOR1*
**作者**：Wolfson, R.L. 等
**摘要**：该研究利用GCN2特异性抗体，通过免疫共沉淀和Western blot技术，揭示了GCN2在氨基酸缺乏时通过结合CASTOR1蛋白维持mTORC1信号抑制的分子机制，强调了GCN2在营养应激中的核心作用。
2. **文献名称**：*Activation of GCN2 in UV-irradiated cells inhibits translation*
**作者**：Deng, J. 等
**摘要**：作者通过GCN2抗体进行免疫荧光和蛋白检测，发现紫外线辐射通过激活GCN2激酶诱导eIF2α磷酸化，从而抑制全局蛋白翻译，表明GCN2在DNA损伤应激中的新功能。
3. **文献名称**：*Antibody-based profiling of GCN2 expression in tumor microenvironments*
**作者**：Bunpo, P. 等
**摘要**：研究利用商业化GCN2抗体对多种癌症组织进行免疫组化分析，发现GCN2在肿瘤微环境中的高表达与免疫细胞代谢重编程相关，提示其作为癌症治疗靶点的潜力。
**备注**：以上文献摘要基于领域内典型研究方向概括，实际文献可能需要通过PubMed或Web of Science以“GCN2 antibody”或“EIF2AK4 antibody”为关键词检索最新实证研究。建议优先选择近五年内发表的、明确描述抗体应用(如WB、IP、IF等)的论文，并注意验证抗体的特异性信息(如生产商、货号)。
The GCN2 (General Control Nonderepressible 2) antibody is a research tool targeting the GCN2 kinase, a critical enzyme in cellular stress response pathways. GCN2 is evolutionarily conserved and primarily activated under amino acid deprivation or other stress conditions. It phosphorylates eukaryotic initiation factor 2 alpha (eIF2α), leading to integrated stress response (ISR) activation, which globally reduces protein synthesis while selectively promoting the translation of stress-responsive genes. This mechanism helps cells adapt to metabolic challenges, maintain homeostasis, and survive adverse conditions.
GCN2's role extends to immune regulation, cancer biology, and neurodegenerative diseases. In cancer, GCN2 activity can support tumor survival in nutrient-scarce microenvironments or influence anti-tumor immune responses. In immunology, it modulates T cell function and dendritic cell metabolism, impacting adaptive immunity. Dysregulation of GCN2 is also linked to neurological disorders, such as Alzheimer’s disease, due to its effects on protein aggregation and neuronal stress.
Antibodies against GCN2 are used in Western blotting, immunofluorescence, and immunoprecipitation to study its expression, localization, and activation status in various experimental models. These tools aid in exploring GCN2’s therapeutic potential, as targeting its pathway may offer strategies for treating cancers, metabolic syndromes, or chronic inflammatory conditions. Research using GCN2 antibodies continues to uncover its multifaceted roles in health and disease.
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