首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素28B(IL-28B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、cellculturesupernats、组织匀浆样本中的IL-28B含量。IL-28B是重要靶点。它属于细胞因子家族，与抗病毒、免疫调节相关。研究机制上，其基因多态性会影响机体对病毒感染的免疫反应，在乙肝、丙肝治疗研究中受关注，有望为相关疾病治疗提供新思路和靶点。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于体外实验环境，能够为病毒感染机制研究、免疫调节通路探索、药物作用效果评估等科研项目提供可靠数据支持，特别是在抗病毒药物开发、慢性炎症性疾病模型构建等基础研究中具有重要应用价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cytokine ZCYTO22 ELISA Kit; IFN-lambda-3 ELISA Kit; IFN-lambda-4 ELISA Kit; IL-28B ELISA Kit; IL-28C ELISA Kit; IL28B ELISA Kit; IL28B_HUMAN ELISA Kit; IL28C ELISA Kit; Interferon lambda-3 ELISA Kit; Interferon lambda-4 ELISA Kit; interleukin 28B (interferon; lambda 3) ELISA Kit; Interleukin 28C ELISA Kit; Interleukin-28B ELISA Kit; Interleukin-28C ELISA Kit
缩写：IL-28B
Uniprot No.：Q8IZI9
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernats, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-28B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 100 | 95-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.2242.2392.2322.047501.5091.5341.5221.337251.0231.0361.0300.84512.50.6880.6990.6940.5096.250.4740.4900.4820.2973.120.3870.3740.3810.1961.560.2810.2970.2890.10400.1840.1860.185 | ||||||
货期：3-5 working days
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