首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素22(IL-22)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-22含量。IL-22是一个重要靶点，它为白细胞介素家族成员，与免疫调节密切相关。其研究机制涉及参与调节组织修复、炎症反应等过程。在多种疾病如自身免疫病、炎症性肠病等的发生发展中发挥作用，是药物研发的潜在方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域对IL - 22表达水平的精准分析；可广泛用于炎症性疾病动物模型（如结肠炎、皮炎或呼吸道感染）的病理机制研究、药物干预效果评价或免疫调控实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-22
Uniprot No.：G3V6X6
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-94 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of rat IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 95-105 | ||||
| EDTA plasma (n=4) | 104 | 92-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.1202.1472.1341.9585001.4931.4861.4901.3142501.0291.1051.0670.8911250.6580.6730.6660.49062.50.4560.4440.4500.27431.20.3460.3580.3520.17615.60.2700.2620.2660.09000.1740.1780.176 | ||||||
货期：3-5 working days
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