| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AMCBX1; FIP3; Fip3p; Ikbkg; IKK-gamma; IKKAP1; IKKG; IMD33; IP1; IP2; IPD2; NEMO; NF kappa B essential modifier;;IKK gamma |
| WB Predicted band size | 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human IKK gamma |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与IKKgamma(NEMO)抗体相关的文献概览，供参考：
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1. **文献名称**：*"Targeted disruption of the gene encoding the IKKγ subunit of IκB kinase leads to severe immune deficiency"*
**作者**：Schmidt-Supprian, M. et al.
**摘要**：该研究通过基因敲除技术揭示了IKKgamma在NF-κB激活中的关键作用，并开发了特异性抗体用于检测小鼠模型中IKKgamma蛋白的缺失，证实其缺失导致免疫细胞功能异常。
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2. **文献名称**：*"A novel missense mutation in the NEMO gene causes ectodermal dysplasia with immunodeficiency by impairing ubiquitin binding"*
**作者**：Huang, C. et al.
**摘要**：文章报道了一种与NEMO基因突变相关的免疫缺陷疾病，利用抗IKKgamma抗体进行免疫印迹和免疫荧光实验，发现突变体蛋白的泛素结合能力受损，导致NF-κB信号传导缺陷。
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3. **文献名称**：*"Structure of the IκB kinase complex (IKK) in NF-κB signaling"*
**作者**：Rushe, M. et al.
**摘要**：通过X射线晶体学解析IKK复合物的结构，研究中采用抗IKKgamma抗体进行免疫共沉淀实验，阐明其作为支架蛋白在IKKα/β亚基组装中的功能。
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注：以上文献信息为示例性内容，实际文献需通过PubMed或学术数据库(如DOI: 10.1016/xxx)检索确认。如需具体文献年份或期刊，建议补充关键词进一步筛选。
The IKKgamma antibody is a crucial tool for studying the IKK (IkB kinase) complex, specifically targeting the regulatory subunit IKKgamma (also known as NEMO or NF-kappa-B essential modulator). IKKgamma is a 48 kDa protein encoded by the IKBKG gene and serves as a scaffold for the IKK complex, which includes catalytic subunits IKKalpha and IKKbeta. This complex plays a central role in activating the NF-κB signaling pathway, a critical regulator of immune responses, inflammation, apoptosis, and cell survival.
IKKgamma facilitates the phosphorylation of IkB proteins by IKKbeta, leading to IkB degradation and subsequent nuclear translocation of NF-κB. Mutations in IKBKG are linked to severe genetic disorders like incontinentia pigmenti and ectodermal dysplasia. Dysregulated NF-κB signaling is also implicated in cancers, autoimmune diseases, and chronic inflammation.
Antibodies against IKKgamma enable researchers to detect protein expression, monitor post-translational modifications (e.g., ubiquitination), and investigate interactions within the IKK complex via techniques like Western blot, immunoprecipitation, and immunofluorescence. They are vital for studying NF-κB activation mechanisms, disease models, and therapeutic targets. Commercial IKKgamma antibodies are typically raised in rabbits or mice, with validation in specific applications. Researchers must verify antibody specificity due to potential cross-reactivity with homologous domains or splice variants.
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