| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAM kinase 2 delta; CAM kinase II delta; CaM kinase II delta subunit; CaM kinase II subunit delta; CAMK 2d; CaMK-II delta subunit; CAMK2D; CAMKI; ;CaMKII delta |
| WB Predicted band size | Calculated MW: 56 kDa ; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CaMKII delta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CaMKIIdelta抗体的3篇代表性文献示例(注：内容为模拟概括，实际文献需通过学术数据库检索)：
1. **文献名称**：*"CaMKIIδ Isoform-Specific Antibody Validation in Cardiac Hypertrophy Models*"
**作者**：Zhang L, et al.
**摘要**：通过Western blot和免疫荧光技术验证了CaMKIIdelta抗体的特异性，发现其在心肌肥厚小鼠模型中能特异性识别CaMKIIδ亚型，并揭示该亚型在压力超负荷下的表达上调与心脏功能障碍相关。
2. **文献名称**：*"Role of CaMKIIδ in Diabetic Cardiomyopathy: Insights from Antibody-Based Inhibition Studies*"
**作者**：Anderson KR, et al.
**摘要**：利用CaMKIIdelta抗体阻断其活性，发现高糖环境下心肌细胞凋亡减少，表明CaMKIIδ通过调控线粒体途径促进糖尿病心肌病进展，抗体特异性通过siRNA对照实验验证。
3. **文献名称**：*"CaMKIIδ Splice Variants in Arrhythmia: Antibody-Driven Isoform Differentiation*"
**作者**：Sato D, et al.
**摘要**：研究通过两种CaMKIIdelta亚型抗体(针对δB和δC变体)区分其在人心房肌中的分布，证实δC亚型在房颤患者中异常聚集，并与钙处理蛋白的磷酸化增强相关。
建议通过PubMed或Google Scholar以关键词“CaMKII delta antibody”、“CaMKIIδ-specific antibody”检索最新文献获取准确信息。
The CaMKIIδ (Calcium/Calmodulin-Dependent Protein Kinase II delta) antibody is a critical tool for studying the delta isoform of CaMKII, a serine/threonine kinase central to calcium signaling pathways. CaMKIIδ is predominantly expressed in the heart, smooth muscle, and brain, where it regulates diverse cellular processes, including gene transcription, ion channel activity, and cell survival/apoptosis. In the heart, CaMKIIδ activation is linked to pathological cardiac remodeling, arrhythmias, and heart failure, making it a therapeutic target.
The CaMKIIδ antibody specifically detects this isoform, distinguishing it from other CaMKII subtypes (α, β, γ) through recognition of unique epitopes in the variable domain or linker region. Researchers use it in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and post-translational modifications (e.g., autophosphorylation at Thr287. indicating activation). Its specificity is validated via knockout controls or isoform-specific siRNA knockdown.
Commercially available antibodies are often raised against synthetic peptides or recombinant proteins corresponding to the C-terminal or regulatory domains of human or mouse CaMKIIδ. Variations in splice isoforms (δB, δC) require careful antibody selection to ensure target specificity. This antibody is pivotal in cardiovascular research, enabling mechanistic studies of CaMKIIδ's role in hypertrophy, fibrosis, and metabolic disorders, as well as screening potential inhibitors for therapeutic development.
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