| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Actin binding protein; ALS18; Epididymis tissue protein Li 184a; PFN 1; Pfn; PFN1; Profilin1; ProfilinI;;Profilin 1 |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Profilin 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Profilin1抗体相关的代表性文献摘要(文献名称及内容为虚构示例，供参考)：
1. **文献名称**：*Profilin1 Antibody Validation in Neurodegenerative Disease Models*
**作者**：Smith J, et al.
**摘要**：本研究验证了Profilin1抗体的特异性，发现其在阿尔茨海默病模型中能有效检测神经元内Profilin1的异常聚集，提示其可作为神经退行性疾病的潜在生物标志物。
2. **文献名称**：*Profilin1 Expression in Breast Cancer: A Novel Therapeutic Target*
**作者**：Chen L, et al.
**摘要**：通过免疫组化与Western blot分析，发现Profilin1在乳腺癌组织中高表达，且其抗体可抑制癌细胞迁移，表明其在肿瘤治疗中的潜在应用价值。
3. **文献名称**：*Profilin1 Interaction with Actin Cytoskeleton: Insights from Knockout Mice*
**作者**：Wang Y, et al.
**摘要**：利用基因敲除小鼠模型和特异性抗体，证实Profilin1通过调控肌动蛋白动力学影响细胞运动，为细胞骨架研究提供了关键工具。
如需真实文献，建议通过PubMed或Google Scholar检索关键词“Profilin1 antibody” + 应用方向(如“cancer”“neurodegeneration”)。
Profilin1 (PFN1) is a ubiquitously expressed actin-binding protein that plays a critical role in regulating cytoskeletal dynamics by promoting actin polymerization and sequestering monomeric actin. It also interacts with phosphoinositides and proline-rich domains of various proteins, influencing cell motility, membrane trafficking, and signal transduction. PFN1 dysregulation is implicated in cancer metastasis, neurodegenerative disorders (e.g., ALS), and immune diseases, making it a key focus in biomedical research.
Antibodies targeting PFN1 are essential tools for studying its expression, localization, and function. These antibodies, often generated in rabbits or mice, recognize specific epitopes within the PFN1 protein and are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect multiple isoforms or post-translationally modified variants. Validation of PFN1 antibodies typically involves knockdown/knockout controls to confirm target specificity.
Research applications include investigating PFN1's role in tumor invasiveness, neuronal cytoskeleton remodeling, and immune cell activation. Aberrant PFN1 expression has been linked to breast cancer progression and ALS pathology, highlighting its therapeutic potential. Recent studies also explore PFN1-antibody complexes as biomarkers for disease diagnosis. Proper antibody selection and validation remain crucial for accurate experimental outcomes in both basic and clinical studies.
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