首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素21(IL-21)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL21含量。IL21，即白细胞介素-21，是一种由CD4+T细胞和自然杀伤T(NKT)细胞产生的细胞因子，属于IL-15/IL-21家族。它通过结合IL-21R受体激活JAK1和JAK3信号，进而调节T、B、NK细胞和DC的增殖与分化，在免疫调节中发挥重要作用。IL21在多种免疫疾病中扮演关键角色，如自身免疫性疾病和癌症。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外定量分析大鼠外周血或组织样本中IL-21的动态变化，可广泛应用于免疫调节机制研究、炎症性疾病动物模型评估或药物干预后免疫指标变化的科研分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il21Interleukin-21 ELISA Kit; IL-21 ELISA Kit
缩写：IL21
Uniprot No.：A3QPB9
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 99-108 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-101 | ||||
| EDTA plasma (n=4) | 92 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6012.5442.5732.4565001.9211.9521.9371.8202501.1981.1221.1601.0431250.7350.7090.7220.60562.50.4250.4590.4420.32531.20.2720.2570.2650.14815.60.1810.1720.1770.06000.1160.1180.117 | ||||||
货期：3-5 working days
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