Rabbit Monoclonal PKA2beta Antibody

The PKA2beta antibody is a crucial tool for studying the protein kinase A (PKA) signaling pathway, specifically targeting the beta isoform of the catalytic subunit (Cβ) of PKA. PKA is a serine/threonine kinase central to intracellular signal transduction, regulating processes like metabolism, gene expression, and cell proliferation. It exists as a tetrameric holoenzyme comprising two regulatory (R) and two catalytic (C) subunits. Upon cAMP binding, the R subunits release active C subunits, which phosphorylate downstream targets. Mammals express multiple C subunit isoforms (Cα, Cβ, Cγ), with Cβ (encoded by *PRKACB*) exhibiting tissue-specific expression, particularly in the brain, testes, and immune cells. The PKA2beta antibody selectively recognizes the Cβ isoform, enabling researchers to distinguish it from other isoforms (e.g., Cα) in experimental settings. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess Cβ expression, localization, and activity in physiological and pathological contexts. Dysregulation of PKA-Cβ has been implicated in neurological disorders, cancer, and metabolic diseases, making this antibody valuable for mechanistic studies. Validated applications, host species (e.g., rabbit, mouse), and clonality (monoclonal/polyclonal) vary by commercial source, requiring careful selection based on experimental needs. Proper controls are essential to ensure specificity, given potential cross-reactivity with homologous isoforms.