首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素18(IL-18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的IL18含量。IL18即白细胞介素18，是一种促炎细胞因子。它在免疫调节中起重要作用，能促进Th1、Th17细胞分化及IFN-γ产生。目前围绕IL18开展的研究聚焦于其在自身免疫病、炎症性疾病等发病机制中的作用，以及开发针对它的治疗策略。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品可广泛应用于大鼠模型中炎症相关疾病机制研究、免疫调控实验验证以及体外细胞因子分泌水平检测等科研场景，为感染性疾病模型、自身免疫性疾病研究等提供可靠的IL-18浓度检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：IL18
Uniprot No.：P97636
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 93-96 | ||||
| EDTA plasma (n=4) | 102 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.3572.3872.3722.218 51.6861.6991.6931.539 2.51.0641.0151.0400.886 1.250.6870.6540.6710.517 0.6250.3820.3980.3900.236 0.3120.2890.2700.2800.126 0.1560.2210.2350.2280.074 00.1510.1570.154 | ||||||
货期：3-5 working days
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