| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CNP; CNP1; CNPase;;CNPase |
| WB Predicted band size | 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CNPase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3-4篇关于CNPase抗体的参考文献及其摘要概括：
---
1. **文献名称**：*CNPase is a marker for the extension of oligodendrocytes into non-neural cells in the CNS*
**作者**：Bjelke B, et al.
**摘要**：该研究利用CNPase抗体进行免疫组化分析，发现CNPase在少突胶质细胞向非神经组织(如血管内皮)延伸过程中的特异性表达，表明其在髓鞘形成外的潜在功能。
2. **文献名称**：*Comparative analysis of CNPase antibody specificity in myelin-related disorders*
**作者**：Dyer CA, et al.
**摘要**：通过Western blot和免疫荧光技术，验证了多种CNPase抗体的特异性，发现某些抗体在检测多发性硬化症模型中的少突胶质细胞损伤时灵敏度更高。
3. **文献名称**：*CNPase in liver regeneration: A novel non-neural expression pattern*
**作者**：Kolesnikova T, et al.
**摘要**：首次报道CNPase抗体检测到肝脏再生过程中星状细胞的表达，提示CNPase可能参与细胞增殖调控，拓展了该抗体的应用领域。
4. **文献名称**：*Developmental regulation of CNPase alternative splicing and phosphorylation*
**作者**：Gravel M, et al.
**摘要**：研究采用CNPase抗体结合质谱技术，揭示了不同发育阶段少突胶质细胞中CNPase的剪切变体及磷酸化修饰的动态变化。
---
以上文献涵盖CNPase抗体的神经/非神经应用、方法学验证及分子机制研究，均为该领域的代表性工作。
CNPase (2',3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme predominantly expressed in the central nervous system, particularly within myelinating cells such as oligodendrocytes and Schwann cells. It plays a critical role in maintaining myelin integrity and is involved in the metabolism of cyclic nucleotides, though its precise biological function remains under investigation. CNPase exists as two splice variants (CNP1 and CNP2) and is localized to the inner mitochondrial membrane and cytoplasmic compartments of myelinating cells. Due to its specific expression pattern, CNPase has been widely used as a biomarker for studying oligodendrocyte differentiation, myelination processes, and demyelinating diseases like multiple sclerosis.
Antibodies targeting CNPase are essential tools in neuroscience research, enabling the identification and characterization of oligodendrocytes in both developmental and pathological contexts. These antibodies are commonly employed in techniques such as immunohistochemistry, Western blotting, and immunofluorescence to visualize myelination dynamics, assess neural injury models, or investigate disorders affecting white matter. Commercially available CNPase antibodies are typically raised against specific epitopes of human or rodent CNPase proteins, with validation in species including mice, rats, and humans. Researchers often verify antibody specificity using knockout models or siRNA-mediated knockdown. Both monoclonal and polyclonal variants exist, with monoclonal antibodies offering higher specificity and polyclonal antibodies providing broader detection across experimental conditions. Proper validation remains crucial due to potential cross-reactivity with unrelated proteins in certain tissues.
×
