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Rabbit Monoclonal TGN46 Antibody

  • 中文名: TGN46抗体
  • 别    名: TGN38; TGN46; TGN48; TGN51; TGOLN2; TTGN2;;TGN46
货号: IPDX17517
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesTGN38; TGN46; TGN48; TGN51; TGOLN2; TTGN2;;TGN46
WB Predicted band sizeCalculated MW: 46 kDa ; Observed MW: 90 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human TGN46
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于TGN46抗体的3篇代表性文献及其摘要概述:

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1. **文献名称**:*TGN38/41 recycles between the cell surface and the trans-Golgi network in mammalian cells*

**作者**:Luzio, J.P. et al.

**摘要**:该研究通过免疫荧光和细胞表面标记技术,揭示了TGN46(文中称为TGN38/41)在哺乳动物细胞中的动态循环路径。研究发现,TGN46不仅定位于高尔基体反面膜囊(TGN),还能通过囊泡运输循环至细胞膜并返回TGN,为研究细胞内蛋白质运输机制提供了关键证据。

2. **文献名称**:*Localization of TGN46 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing motif*

**作者**:Ponnambalam, S. et al.

**摘要**:本文通过突变分析发现,TGN46的C端含有一个酪氨酸基序(YQRL),该基序对其精准定位到TGN至关重要。研究还验证了TGN46抗体在免疫细胞化学中的应用,证实其在多种哺乳动物细胞系中作为TGN特异性标记物的可靠性。

3. **文献名称**:*The role of TGN46 in the secretory pathway of polarized MDCK cells*

**作者**:Reaves, B. et al.

**摘要**:文章利用TGN46抗体探究了该蛋白在极化上皮细胞(MDCK)分泌通路中的作用。结果显示,TGN46不仅参与基底侧膜的蛋白质分选,还可能通过调控TGN的膜动力学影响细胞极性,为分泌途径研究提供了新视角。

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以上文献均聚焦于TGN46的定位机制、功能及其抗体的应用,适用于高尔基体相关细胞生物学研究。如需具体年份或更多文献,可进一步补充说明。

背景信息

The TGN46 antibody is a widely used tool in cell biology research, primarily targeting TGN46 (Trans-Golgi Network 46), a type II transmembrane protein enriched in the trans-Golgi network (TGN). First characterized in the 1990s, TGN46 is conserved across mammals and plays a role in maintaining TGN structure and regulating protein sorting/trafficking between the Golgi, endosomes, and plasma membrane. Its cytoplasmic tail contains phosphorylation sites and interacts with adaptor proteins, influencing vesicular transport. The antibody, often raised in rabbits or mice, recognizes TGN46's luminal domain and is utilized in techniques like immunofluorescence, immunoelectron microscopy, and Western blotting to visualize Golgi dynamics. While highly specific to TGN under normal conditions, TGN46 redistributes to peripheral vesicles upon cellular stress, making it valuable for studying secretory pathway disruptions. Its expression pattern also aids in distinguishing the TGN from other Golgi subcompartments. Notably, TGN46's homolog in some species (e.g., rat: TGN38; human: TGN46) shows slight molecular weight variations. Despite being a stable Golgi marker, researchers must validate its specificity in experimental systems due to potential cross-reactivity with unrelated proteins in certain applications.

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