| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VAMP8; EDB; Endobrevin; VAMP-8;;VAMP8 |
| WB Predicted band size | Calculated MW: 11 kDa ; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VAMP8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于VAMP8抗体的3篇文献摘要信息：
1. **文献名称**：*"VAMP8 is a vesicle SNARE that regulates fusion of recycling endosomes during exocytosis in neutrophils"*
**作者**：Brumell, J.H., et al.
**摘要**：该研究利用VAMP8特异性抗体，揭示了VAMP8在中性粒细胞脱颗粒过程中的关键作用，证明其通过调控循环内体与质膜的融合参与炎症反应。
2. **文献名称**：*"VAMP8 mediates platelet secretion and hemostasis through selective granule release"*
**作者**：Ren, Q., et al.
**摘要**：通过VAMP8抗体阻断实验，作者发现VAMP8选择性介导血小板致密颗粒的分泌，而在α颗粒释放中作用有限，表明其在止血功能中的特异性调控机制。
3. **文献名称**：*"Role of VAMP8 in lysosomal exocytosis and autophagosome-lysosome fusion"*
**作者**：Furuta, N., et al.
**摘要**：研究使用VAMP8抗体抑制实验，证明VAMP8在自噬体-溶酶体融合及溶酶体胞吐中的必要性，为溶酶体相关疾病提供了分子机制依据。
(注：以上文献信息为示例，具体内容需根据实际论文调整。)
VAMP8 (Vesicle-associated membrane protein 8), also known as endobrevin, is a member of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) family, which plays a critical role in intracellular membrane fusion events. It is primarily involved in facilitating vesicle trafficking, particularly in endosomal, lysosomal, and secretory pathways. VAMP8 mediates the fusion of vesicles with target membranes by forming a complex with syntaxin and SNAP-25/23 homologs, enabling processes like exocytosis, autophagy, and platelet secretion.
VAMP8 antibodies are essential tools for studying its expression, localization, and function in cellular mechanisms. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect VAMP8 in various tissues and cell types. Research has linked VAMP8 to physiological and pathological conditions, including neurotransmitter release, immune response regulation, and cancer progression. Dysregulation of VAMP8 has been implicated in diseases like diabetes, neurodegenerative disorders, and certain cancers, making it a potential biomarker or therapeutic target.
High-quality VAMP8 antibodies exhibit specificity for their target epitope, often validated through knockout controls or siRNA knockdowns. They are crucial for elucidating the molecular mechanisms of vesicle trafficking and their implications in health and disease.
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