首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素16(IL-16)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL16含量。IL16作为一个靶点，其背景上是具有免疫调节功能的细胞因子。在研究机制方面，它能与CD4受体结合，参与T淋巴细胞等的活化、增殖与迁移过程，在免疫相关疾病研究中受关注，为疾病治疗策略开发提供潜在方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于大鼠炎症机制研究、免疫相关疾病模型评估及药物干预效果分析等科研领域；可满足细胞因子网络研究、免疫微环境分析等实验需求，尤其适用于神经科学、免疫学及药理学领域的体外样本检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-16
Uniprot No.：G5BEZ3
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 98-107 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of rat IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 88 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.7572.6562.7072.524 10002.2232.1222.1731.990 5001.4881.4671.4781.295 2500.9370.9260.9320.749 1250.5890.5780.5840.401 62.50.4250.4140.4200.237 31.250.3050.2970.3010.118 00.1830.1820.183 | ||||||
货期：3-5 working days
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