首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素15(IL-15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IL15含量。IL15是一种多效性细胞因子，在免疫系统中发挥重要作用。它通过与其受体结合，激活下游信号通路，促进T细胞、B细胞和NK细胞的增殖和分化。IL15在肿瘤免疫治疗中具有潜力，能够激活抗肿瘤免疫，成为癌症治疗的新靶点。试剂盒检测范围为1.56 pg/mL-100 pg/mL，炎症反应、免疫性疾病模型构建、药物干预效果评估、免疫调控机制研究、感染性疾病模型分析及生物标志物筛选、基础科研与转化医学研究中对IL - 15动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
缩写：IL15
Uniprot No.：P97604
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-104 | |||||
回收率：
| The recovery of rat IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-101 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.5112.5672.5392.383501.7731.7831.7781.622251.1061.1541.1300.97412.50.7040.7250.7150.5596.250.4150.4310.4230.2673.120.2870.2970.2920.1361.560.2140.2310.2230.06700.1550.1570.156 | ||||||
货期：3-5 working days
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