| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CollagenX; COL10A1; COAA1; Collagen type X alpha 1;;Collagen X alpha 1 |
| WB Predicted band size | 66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Collagen X alpha 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Collagen X抗体的3篇参考文献，涵盖其在骨骼发育、疾病模型及检测技术中的应用：
1. **文献名称**：*Collagen X deposition regulates hypertrophic chondrocyte differentiation in mouse growth plate*
**作者**：Kwan AP, Dickson IR, Freemont AJ, Grant ME
**摘要**：该研究通过免疫组化技术结合Collagen X特异性抗体，揭示了Collagen X在小鼠生长板肥大软骨细胞分化中的关键作用，并验证了其在钙化前基质中的动态沉积过程。
2. **文献名称**：*Monoclonal antibodies against type X collagen for studying skeletal disorders*
**作者**：Schmid TM, Linsenmayer TF
**摘要**：研究团队开发了针对Collagen X的单克隆抗体，验证其特异性后应用于鸡和小鼠模型，发现其在骨关节炎和骨软骨发育不良中的异常表达，为疾病机制研究提供工具。
3. **文献名称**：*Collagen X as a biomarker of endochondral ossification in zebrafish models*
**作者**：Gistelinck C, Witten PE, Huysseune A
**摘要**：利用抗Collagen X抗体的免疫荧光技术，研究斑马鱼模型内软骨内骨化过程，发现其在骨骼修复中的表达动态，提示其作为骨化进程的生物标志物潜力。
这些文献展示了Collagen X抗体在定位、机制研究及疾病诊断中的广泛应用。
Collagen X antibody is a specialized tool used to detect and study type X collagen, a key component in vertebrate extracellular matrices. Collagen X, encoded by the *COL10A1* gene, is a short-chain, network-forming collagen primarily synthesized by hypertrophic chondrocytes during endochondral ossification—a critical process in skeletal development and long bone growth. Unlike other collagens, it forms hexagonal networks that stabilize the matrix in hypertrophic cartilage zones, facilitating mineralization and vascular invasion.
Mutations in *COL10A1* are linked to skeletal disorders like Schmid metaphyseal chondrodysplasia (SMCD), characterized by growth plate abnormalities and short stature. Researchers use Collagen X antibodies to investigate these pathologies, track chondrocyte differentiation in vitro, and assess cartilage repair strategies in tissue engineering. In diagnostics, these antibodies help identify Collagen X overexpression in certain cancers, such as chondrosarcoma or breast cancer, where its ectopic expression may correlate with metastasis.
Antibodies targeting Collagen X are typically generated in hosts like rabbits or mice, validated via Western blot, immunohistochemistry, or immunofluorescence. Their specificity enables precise localization in tissues, aiding studies on skeletal biology, osteoarthritis, and tumor microenvironments. Recent advances also explore their therapeutic potential, such as blocking Collagen X-mediated pathways in fibrotic diseases. Overall, Collagen X antibodies serve as indispensable tools bridging basic research, clinical diagnostics, and targeted therapies.
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