| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LAMA3; Laminin 5 alpha3; E170; LAMNA; LOCS; laminin alpha 3; Laminin A3;;Laminin alpha 3 |
| WB Predicted band size | 367 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Laminin alpha 3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Laminin α3(LAMA3)抗体的3篇参考文献示例，涵盖其在疾病研究和诊断中的应用：
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1. **文献名称**: "Laminin-332 in squamous cell carcinoma: Biological roles and therapeutic potential"
**作者**: Hamill K.J. et al.
**摘要**: 该研究探讨了Laminin-332(含α3链)在皮肤鳞状细胞癌中的表达模式，利用特异性抗体揭示了其与肿瘤侵袭性的关联，并提出靶向LAMA3可能抑制癌细胞迁移。
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2. **文献名称**: "Autoantibodies against laminin α3 in mucous membrane pemphigoid"
**作者**: Lazarova Z. et al.
**摘要**: 通过LAMA3抗体检测，发现黏膜类天疱疮患者血清中存在针对Laminin α3链的自身抗体，证实其在疾病诊断中的生物标志物价值。
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3. **文献名称**: "Laminin α3 chain dysfunction in junctional epidermolysis bullosa"
**作者**: Pulkkinen L. et al.
**摘要**: 研究利用抗LAMA3抗体进行免疫荧光分析，揭示交界型大疱性表皮松解症患者皮肤基底膜中Laminin-332的α3链缺失，明确基因突变导致的蛋白功能异常。
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*注：上述文献为示例，实际引用时需核实具体来源及细节。若需精准文献，建议通过PubMed或Web of Science以“LAMA3 antibody”、“anti-laminin alpha3”等关键词检索。*
Laminin-5 (laminin-332), a key component of the basement membrane, is a heterotrimeric glycoprotein composed of α3. β3. and γ2 chains. The α3 subunit, encoded by the *LAMA3* gene, plays a critical role in epithelial-stromal adhesion, cell differentiation, and signaling. Laminin-5α3 antibodies specifically target the α3 chain, which is essential for anchoring epithelial cells to the underlying extracellular matrix. These antibodies are widely used in research to study tissue integrity, wound healing, and diseases linked to basement membrane dysfunction, such as epidermolysis bullosa and certain cancers.
In clinical diagnostics, laminin-5α3 antibodies help identify pathological conditions like mucous membrane pemphigoid and junctional epidermolysis bullosa (JEB), where genetic mutations in *LAMA3* disrupt laminin-332 assembly, leading to fragile skin and mucosal blistering. Additionally, altered laminin-5α3 expression is associated with tumor progression, as its cleavage by proteases like MMP-2/9 facilitates cancer cell invasion and metastasis.
Antibodies against laminin-5α3 are employed in techniques like immunohistochemistry, immunofluorescence, and Western blotting to visualize protein distribution, assess tissue pathology, and validate disease models. Their specificity makes them valuable tools for both mechanistic studies and therapeutic target exploration in epithelial biology and oncology.
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