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Human Interleukin 10,IL-10 ELISA KIT

  • 中文名： 人白介素10(IL-10)ELISA KIT
货号: IPD-E04593h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素10(IL-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL-10（白细胞介素-10）是一种多效性细胞因子，主要由单核细胞、T细胞（主要是Th2细胞）等产生。它通过与其受体结合，抑制Th1细胞产生细胞因子，发挥免疫抑制功能。IL-10在免疫调节和炎症中具有重要作用，其信号通路涉及JAK1和Tyk2激酶以及STAT3信号转导。试剂盒检测范围为31.25 pg/ml- 2000 pg/ml，灵敏度为7.8 pg/ml。该产品适用于科研领域中对炎症反应机制、免疫调节通路或疾病模型中IL - 10动态变化的研究，例如在自身免疫疾病模型、肿瘤免疫治疗评估或感染性疾病研究中定量分析生物标志物表达水平，为体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CSIF ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; GVHDS ELISA Kit; IL 10 ELISA Kit; IL-10 ELISA Kit; IL10 ELISA Kit; IL10_HUMAN ELISA Kit; IL10A ELISA Kit; Interleukin 10 ELISA Kit; Interleukin-10 ELISA Kit; MGC126450 ELISA Kit; MGC126451 ELISA Kit; T-cell growth inhibitory factor ELISA Kit; TGIF ELISA Kit
  • 缩写：
    IL10
  • Uniprot No.：
    P22301
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/ml - 2000 pg/ml
  • 灵敏度：
    7.8 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    86-98


    1:2

    Average %

    92


    Range %

    88-100


    1:4

    Average %

    90


    Range %

    86-98


    1:8

    Average %

    90


    Range %

    84-96


  • 回收率：

    The recovery of human IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-101


    EDTA plasma (n=4)

    91

    86-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.715

    2.8334

    2.774

    2.616


    1000

    2.243

    2.1667

    2.205

    2.046


    500

    1.438

    1.4280

    1.433

    1.275


    250

    0.900

    0.9290

    0.914

    0.756


    125

    0.552

    0.5321

    0.542

    0.384


    62.5

    0.410

    0.4253

    0.418

    0.259


    31.25

    0.294

    0.2840

    0.289

    0.131


    0

    0.162

    0.1550

    0.158




  • 货期：
    3-5 working days

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