| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mammary gland factor (STAT5A); Signal transducer and activator of transcription 5A; Stat 5a; STAT 5b; STAT5A; Transcription factor STAT5B;;STAT5A/B |
| WB Predicted band size | Calculated MW: 91,90 kDa ; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human STAT5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于STAT5A/B抗体的代表性文献(简要概括，非完整引用)：
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1. **文献名称**: *"STAT5 activation is critical for prolactin-mediated mammary carcinogenesis"*
**作者**: Wagner KU 等
**摘要**: 研究STAT5A/B在乳腺癌中的功能，通过Western blot和免疫组化验证特异性抗体，证明STAT5的持续激活促进肿瘤发生。
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2. **文献名称**: *"Differential roles of STAT5A and STAT5B in T cell development and function"*
**作者**: Snow JW 等
**摘要**: 比较STAT5A/B在T细胞中的差异，使用特异性抗体进行ChIP-seq和流式细胞术，揭示STAT5B对免疫反应调控更关键。
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3. **文献名称**: *"STAT5 mutations in hematologic malignancies promote pathogenesis by disrupting autoinhibition"*
**作者**: Bandapalli OR 等
**摘要**: 分析STAT5A/B突变对白血病的影响，通过免疫沉淀(IP)和磷酸化抗体检测，发现突变导致组成性激活。
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4. **文献名称**: *"IL-2-induced STAT5 signaling in regulatory T cells limits autoimmune inflammation"*
**作者**: Chinen T 等
**摘要**: 研究IL-2通过STAT5A/B调控Treg细胞功能，使用多种抗体(包括p-STAT5)验证信号通路，为自身免疫疾病提供机制依据。
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**备注**：以上为示例性概括，实际引用需根据具体研究需求检索PubMed或Google Scholar获取完整文献。
**Background of STAT5A/B Antibodies**
STAT5A and STAT5B are closely related transcription factors belonging to the STAT (Signal Transducer and Activator of Transcription) family, which mediate cellular responses to cytokines, growth factors, and hormones. They are encoded by separate genes (*STAT5A* and *STAT5B*) but share ~96% amino acid sequence identity, leading to overlapping yet distinct roles in signaling. STAT5A is critical in mammary gland development and lactation, while STAT5B is more involved in growth hormone signaling and immune regulation. Both proteins are activated via phosphorylation (e.g., by JAK kinases), forming dimers that translocate to the nucleus to regulate genes controlling proliferation, survival, and differentiation.
Antibodies targeting STAT5A/B are essential tools for studying their expression, activation, and localization in cells and tissues. These antibodies often recognize conserved regions, such as the N-terminus or phosphorylated tyrosine residues (e.g., Tyr694/699), enabling detection of total STAT5 or its active form (p-STAT5). Specific clones may differentiate STAT5A from STAT5B, but many commercial antibodies cross-react due to high homology. Applications include Western blotting, immunohistochemistry, flow cytometry, and ChIP assays.
STAT5 dysregulation is linked to cancers, immune disorders, and hematologic diseases. For example, constitutive STAT5 activation drives leukemogenesis and solid tumor progression. Thus, STAT5A/B antibodies are vital for mechanistic studies and therapeutic targeting. Researchers must validate antibody specificity using knockout controls or isoform-specific assays to avoid misinterpretation. Overall, these reagents underpin investigations into STAT5 biology and its translational relevance.
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