| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GOK; SIM; STIM 1; Stim1 stromal interaction molecule 1; STIM1L; Stromal interaction molecule 1; ;Stromal interaction molecule 1 |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Stromal interaction molecule 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与STIM1抗体相关的文献摘要概括：
1. **文献名称**：*STIM1. an essential and conserved component of store-operated Ca²⁺ channel function*
**作者**：Roos J et al.
**摘要**：该研究首次阐明了STIM1作为内质网钙离子传感器的关键作用，开发了特异性STIM1抗体用于检测其在细胞内的定位，证实STIM1通过钙库耗竭激活细胞膜上的钙通道。
2. **文献名称**：*STIM1 clusters and activates CRAC channels via direct binding of a cytosolic domain to Orai1*
**作者**：Park CY et al.
**摘要**：利用STIM1抗体进行免疫共沉淀和荧光定位实验，揭示了STIM1与Orai1蛋白的相互作用机制，证明STIM1在钙信号传导中通过形成多聚体调控CRAC通道活性。
3. **文献名称**：*Antibody-based characterization of STIM1 expression in human tumors*
**作者**：Chen YF et al.
**摘要**：研究通过商业化STIM1抗体对多种癌症组织进行免疫组化分析，发现STIM1在乳腺癌和结肠癌中高表达，且与患者预后不良相关，提示其作为肿瘤标志物的潜力。
注：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar以“STIM1 antibody”为关键词检索近年高被引论文获取准确信息。
STIM1 (Stromal Interaction Molecule 1) is a key transmembrane protein involved in calcium (Ca²⁺) signaling, primarily acting as a Ca²⁺ sensor within the endoplasmic reticulum (ER). It plays a central role in store-operated calcium entry (SOCE), a process critical for maintaining intracellular Ca²⁺ homeostasis. When ER Ca²⁺ stores are depleted, STIM1 undergoes conformational changes, clusters at ER-plasma membrane junctions, and activates ORAI1 channels to mediate extracellular Ca²⁺ influx. This mechanism regulates diverse physiological processes, including immune cell activation, muscle contraction, and gene transcription.
STIM1 antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies target specific epitopes of the STIM1 protein, enabling applications like Western blotting, immunofluorescence, flow cytometry, and immunoprecipitation. Researchers use them to investigate STIM1's role in diseases such as immunodeficiency syndromes, cardiovascular disorders, and cancers, where dysregulated Ca²⁺ signaling is implicated. For instance, STIM1 mutations or altered expression have been linked to tubular aggregate myopathy, thrombocytopenia, and tumor progression.
High-quality STIM1 antibodies are validated for specificity, often using knockout cell lines or tissues as controls. Commercial antibodies vary in host species, clonality (monoclonal/polyclonal), and conjugation formats, allowing flexibility in experimental design. Proper validation ensures reliable detection of STIM1's dynamic behavior during SOCE, aiding mechanistic studies of Ca²⁺-dependent cellular responses.
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