首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人免疫球蛋白M(IgM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的IgM含量。试剂盒检测范围为4.68 ng/mL-300 ng/mL，灵敏度为1.17 ng/mL。该产品适用于免疫学基础研究、感染性疾病模型建立、疫苗免疫效果评估等科研场景，可定量分析体液或组织样本中的IgM水平变化，为炎症反应、B细胞功能研究及免疫相关疾病机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IgM
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：4.68 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5000 | Average % | 86 | ||||
| Range % | 80-94 | |||||
| 1:10000 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:20000 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:40000 | Average % | 94 | ||||
| Range % | 86-101 | |||||
回收率：
| The recovery of human IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-101 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 3002.7532.7692.7612.641 1502.4042.4282.4162.296 751.9051.9111.9081.788 37.51.2811.2931.2871.167 18.750.7260.7290.7280.608 9.370.4650.4720.4690.349 4.680.2880.2940.2910.171 00.1210.1190.120 | ||||||
货期：3-5 working days
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