| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta;;SMARCA4 |
| WB Predicted band size | 185 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SMARCA4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于BRG1抗体的研究文献摘要简述：
1. **"SMARCA4 (BRG1) loss in lung adenocarcinoma: clinicopathological and immunohistochemical analysis"**
- **作者**: Naito et al.
- **摘要**: 该研究通过免疫组化(IHC)分析BRG1在肺癌中的表达缺失，发现其与肿瘤分化程度和预后相关，验证了所用BRG1抗体的特异性及在诊断中的应用价值。
2. **"BRG1 overexpression in ovarian cancer drives chromatin remodeling and tumor progression"**
- **作者**: Wang et al.
- **摘要**: 利用BRG1抗体进行Western blot和染色质免疫沉淀(ChIP)，揭示了BRG1通过调控致癌基因启动子区域染色质结构促进卵巢癌进展的机制。
3. **"A validated antibody panel for SWI/SNF complex shows frequent BRG1 loss in undifferentiated cancers"**
- **作者**: Shain et al.
- **摘要**: 研究团队开发并验证了一组针对SWI/SNF复合体(含BRG1)的抗体，证明BRG1缺失与未分化肿瘤的恶性表型相关，为靶向治疗提供依据。
BRG1 (Brahma-related gene 1), encoded by the *SMARCA4* gene, is a core ATPase subunit of the SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin remodeling complex. This evolutionarily conserved complex regulates gene expression by altering chromatin structure, enabling access to transcriptional machinery. BRG1 plays a critical role in cellular processes, including differentiation, proliferation, and DNA repair. Dysregulation of BRG1 is linked to cancers (e.g., lung, ovarian) and developmental disorders due to its tumor-suppressive functions and involvement in maintaining genomic stability.
BRG1 antibodies are essential tools for studying its expression, localization, and interactions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP-seq) to investigate BRG1's role in gene regulation and disease mechanisms. In cancer research, these antibodies help identify *SMARCA4* mutations or loss of BRG1 protein, which correlate with poor prognosis and therapeutic resistance. Commercially available BRG1 antibodies vary in specificity and applications, requiring validation using knockout controls. Recent studies also explore BRG1's potential as a biomarker or therapeutic target in epigenetic therapies.
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