| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CK 4; CK-4; CYK4; Cytokeratin4; FLJ31692; K4; Keratin 4; Keratin; Keratin type II cytoskeletal 4; Keratin4; Krt4; type II cytoskeletal 4; Type-II keratin Kb4;;Cytokeratin 4 |
| WB Predicted band size | Calculated MW: 56 kDa ; Observed MW: 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cytokeratin 4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Cytokeratin 4(CK4)抗体的模拟参考文献示例(仅供格式参考，建议通过学术数据库验证具体内容)：
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1. **文献名称**: "Differential expression of cytokeratin 4 in normal and malignant squamous epithelia: A potential diagnostic marker"
**作者**: Moll, R., et al.
**摘要概要**: 该研究通过免疫组化分析比较CK4在正常食道、口腔黏膜与鳞状细胞癌中的表达，发现CK4在正常复层上皮中高表达，而在癌变组织中显著下调，提示其可能作为鳞癌诊断的辅助标记物。
2. **文献名称**: "Validation of a novel monoclonal antibody for cytokeratin 4 in human tissue samples"
**作者**: Smith, J., & Patel, K.
**摘要概要**: 研究团队开发了一种特异性抗CK4的单克隆抗体，并通过Western blot和免疫荧光验证其在多种上皮组织中的反应性，证实其在病理诊断中的应用潜力。
3. **文献名称**: "Cytokeratin 4 as a biomarker for Barrett’s esophagus progression"
**作者**: Lee, H., et al.
**摘要概要**: 分析CK4在Barrett食管及其向腺癌转化过程中的表达变化，发现CK4缺失与上皮异型增生程度相关，可能用于监测疾病进展。
4. **文献名称**: "Role of cytokeratin 4 in maintaining epithelial barrier integrity: Insights from 3D cell culture models"
**作者**: Zhang, Y., et al.
**摘要概要**: 利用3D上皮模型探究CK4在上皮屏障功能中的作用，发现CK4缺失导致细胞间连接破坏，提示其在黏膜防御中的关键角色。
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**建议**：访问 **PubMed**(https://pubmed.ncbi.nlm.nih.gov)或 **Google Scholar**，使用关键词“Cytokeratin 4 antibody”、“CK4 immunohistochemistry”检索最新文献，并筛选高被引研究以获取权威信息。
Cytokeratin 4 (CK4) is a member of the cytokeratin family, a class of intermediate filament proteins essential for maintaining the structural integrity of epithelial cells. As a type I cytokeratin (acidic), CK4 typically pairs with type II cytokeratin 13 (CK13) to form heteropolymeric filaments. It is predominantly expressed in non-keratinized stratified squamous epithelia, such as those lining the oral mucosa, esophagus, and vaginal epithelium, as well as in certain glandular tissues. CK4 plays a critical role in mechanical resilience, cell signaling, and epithelial differentiation.
CK4-specific antibodies are widely used in research and diagnostics to identify epithelial cell types and study tissue-specific pathologies. In immunohistochemistry (IHC), these antibodies help delineate epithelial origin in tumors, aiding in cancer subtyping and metastatic tumor tracing. For instance, CK4 expression patterns can differentiate between squamous cell carcinomas and adenocarcinomas. Additionally, CK4 antibodies contribute to understanding epithelial disorders like Barrett's esophagus or lichen planus, where CK4 expression may be altered.
Most commercially available CK4 antibodies are monoclonal, ensuring high specificity and reproducibility. Validation often includes Western blotting and IHC on tissues with known CK4 expression. Dysregulation of CK4 has been linked to epithelial-mesenchymal transition in cancers, highlighting its potential as a biomarker. However, interpretation requires context, as cytokeratin profiles vary across tissues and disease states.
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