| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CSDD2; FLJ16517; LIN28B; Protein lin-28 homolog B;;Lin 28B |
| WB Predicted band size | Calculated MW: 27 kDa ; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Lin 28B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Lin28B抗体的3篇代表性文献示例(注：内容基于领域知识模拟，建议核实真实文献)：
1. **文献名称**：*Lin28B promotes hepatocellular carcinoma progression via antibody-validated protein overexpression*
**作者**：Zhang Y, et al.
**摘要**：研究通过Western blot和免疫组化(使用Lin28B特异性抗体)证实Lin28B在肝癌组织中高表达，其表达水平与患者预后不良相关，机制涉及let-7 miRNA抑制和癌基因通路激活。
2. **文献名称**：*Lin28B antibody-based detection reveals its role in neuroblastoma stemness maintenance*
**作者**：Thompson H, et al.
**摘要**：利用商业化Lin28B单克隆抗体进行流式细胞术和免疫荧光分析，发现Lin28B在神经母细胞瘤干细胞中高表达，敲低后显著抑制肿瘤球形成和体内成瘤能力。
3. **文献名称**：*Development of a high-affinity Lin28B monoclonal antibody for translational research*
**作者**：Chen L, et al.
**摘要**：报道一种新型兔源Lin28B单克隆抗体的开发与验证，该抗体在ELISA、免疫沉淀(IP)及组织芯片中表现出高特异性，成功应用于结直肠癌临床样本的定量蛋白分析。
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**提示**：实际文献可通过PubMed/Google Scholar检索关键词“Lin28B antibody”、“Lin28B immunohistochemistry”或结合特定疾病名称(如“Lin28B cancer”)获取，注意选择近5年研究以反映最新进展。
Lin28B is a RNA-binding protein belonging to the Lin28 family, alongside its homolog Lin28A. It plays critical roles in embryonic development, stem cell pluripotency, and metabolism by regulating gene expression at post-transcriptional levels. A key mechanism involves binding to precursor microRNAs (e.g., let-7 family) or mRNAs to block their maturation or modulate translation, thereby influencing cellular differentiation, proliferation, and oncogenesis. Dysregulation of Lin28B is strongly associated with cancer progression, poor prognosis, and therapeutic resistance in various malignancies, including neuroblastoma, hepatocellular carcinoma, and breast cancer.
Lin28B antibodies are essential tools for detecting and quantifying Lin28B protein levels in research and diagnostics. These antibodies enable applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study its expression patterns, subcellular localization, and interactions with RNA targets. Due to Lin28B's role in cancer stemness and metastasis, its antibodies are widely used in oncology research to explore biomarkers and therapeutic targets. Validation of Lin28B antibodies includes specificity testing via knockout cell lines or siRNA knockdown. Their utility extends to developmental biology, regenerative medicine, and studies of metabolic disorders, underscoring Lin28B's multifaceted biological significance.
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