| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p85; p85 beta; P85B; Phosphatidylinositol 3 kinase; PI3 kinase p85 beta subunit; PI3K; PIK3R 2;;PI3 kinase p85 beta |
| WB Predicted band size | 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human PI3 kinase p85 beta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于PI3K p85β抗体的参考文献及其核心内容概括：
1. **文献名称**：*Regulation of PI3K signaling in cancer: mechanisms and therapeutic implications*
**作者**：Fruman DA, et al.
**摘要**：该研究分析了PI3K p85β亚基在肿瘤中的调控作用，通过特异性抗体检测发现其在乳腺癌中高表达，并证实其与PI3K-AKT信号通路的异常激活相关。
2. **文献名称**：*Distinct roles of the phosphatidylinositol 3-kinase regulatory subunits p85α and p85β in cell signaling*
**作者**：Cuevas BD, et al.
**摘要**：作者利用p85β特异性抗体进行免疫沉淀和Western blot，揭示了p85β相较于p85α在胰岛素信号传导中的独特功能，强调其亚基特异性调控机制。
3. **文献名称**：*PI3K p85β regulates autophagic flux through subcellular localization control*
**作者**：Yang H, et al.
**摘要**：通过免疫荧光技术结合p85β抗体，研究发现p85β亚基通过影响自噬相关蛋白的细胞器定位，调控自噬过程，为癌症代谢研究提供新视角。
注：以上文献为示例性概括，实际引用需核对具体研究细节。建议通过PubMed或Google Scholar以关键词“PI3K p85β antibody”“PIK3R2 antibody”检索最新应用文献。
The PI3K p85β antibody is a research tool designed to detect the regulatory subunit p85β of class IA phosphatidylinositol 3-kinases (PI3Ks). PI3Ks are lipid kinases critical for intracellular signal transduction, regulating cell growth, survival, metabolism, and motility. The p85β subunit (encoded by *PIK3R2*) forms a heterodimer with catalytic subunits (e.g., p110α, β, δ) to stabilize the kinase and recruit it to activated receptor tyrosine kinases or adaptor proteins. While p85α (encoded by *PIK3R1*) is the predominant regulatory isoform, p85β shares structural homology but exhibits distinct expression patterns and functional roles, particularly in tissues like the liver and brain.
Antibodies targeting p85β are used to study its tissue-specific signaling contributions, often in comparison to p85α. They help elucidate p85β’s unique interactions with upstream regulators (e.g., IRS-1. growth factor receptors) and downstream effectors (e.g., AKT/mTOR). Dysregulation of p85β is implicated in insulin resistance, cancer progression, and neurological disorders. For example, p85β overexpression has been linked to enhanced PI3K activity in certain tumors, while mutations may disrupt normal metabolic signaling.
These antibodies are validated for applications like Western blotting, immunohistochemistry, and co-immunoprecipitation. Specificity is crucial, as cross-reactivity with p85α or other isoforms can confound results. Researchers rely on p85β-specific antibodies to dissect isoform-selective mechanisms in disease models, aiding therapeutic development for PI3K pathway-targeted treatments.
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