| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DGCR8; DGCRK6; Gy1 ; C22orf12; D16Wis2; pasha;;DGCR8 |
| WB Predicted band size | Calculated MW: 86 kDa ; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human DGCR8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DGCR8抗体的3篇代表性文献的简要信息(注：文献信息为模拟示例，实际引用需以真实文献为准)：
1. **文献名称**: "DGCR8 is essential for microRNA biogenesis and silencing in embryonic stem cells"
**作者**: Wang Y, et al.
**摘要**: 本研究利用DGCR8特异性抗体验证了DGCR8蛋白在胚胎干细胞中的表达及定位，发现其缺失导致microRNA加工异常，并影响干细胞多能性维持。
2. **文献名称**: "A novel role for DGCR8 in psychiatric disorders linked to 22q11.2 deletion"
**作者**: Stark KL, et al.
**摘要**: 通过Western blot和免疫组化实验，作者使用DGCR8抗体发现22q11.2缺失综合征患者脑组织中DGCR8蛋白水平降低，提示其与神经发育异常相关。
3. **文献名称**: "Interaction between DGCR8 and Drosha revealed by co-immunoprecipitation assays"
**作者**: Lee Y, et al.
**摘要**: 该研究利用DGCR8抗体进行免疫共沉淀实验，证实DGCR8与Drosha形成复合体，并解析了其在microRNA初级转录本加工中的协同作用。
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如需真实文献，建议在PubMed或Google Scholar中搜索关键词 "DGCR8 antibody" + "microRNA" / "co-immunoprecipitation" / "knockout" 等，筛选近年高引论文。
DGCR8 (DiGeorge Syndrome Critical Region Gene 8) is a key protein involved in the early stages of microRNA (miRNA) biogenesis. It forms a heterodimeric complex with the RNase III enzyme Drosha, known as the Microprocessor complex, which cleaves primary miRNA transcripts (pri-miRNAs) into precursor miRNAs (pre-miRNAs) in the nucleus. This step is critical for miRNA maturation and subsequent gene regulation. DGCR8 recognizes pri-miRNA structures through its RNA-binding domains and stabilizes Drosha for precise cleavage.
Antibodies targeting DGCR8 are essential tools for studying miRNA processing mechanisms, protein localization, and expression dynamics. They are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate DGCR8's interactions, cellular distribution, and regulatory roles. Researchers also employ these antibodies to explore DGCR8 dysfunction in diseases, such as DiGeorge syndrome (linked to 22q11.2 deletions), neurodevelopmental disorders, and cancers, where miRNA dysregulation is implicated.
Validated DGCR8 antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and undergo rigorous testing for specificity (e.g., using knockout cell lines). Their applications span basic research on RNA biology and translational studies aiming to dissect miRNA-related pathologies or therapeutic targets.
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