| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIST1H2AB; Histone H2A type 1-B/E; Histone H2A.2; Histone H2A/a; Histone H2A/m; H2AFM;;Histone H2A.Z |
| WB Predicted band size | Calculated MW: 14 kDa ; Observed MW: 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H2A.Z |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Histone H2A.Z抗体的参考文献及其简要摘要：
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1. **文献名称**：*"H2A.Z: a molecular rheostat for transcriptional control"*
**作者**：Zlatanova J, Thakar A (2007)
**摘要**：该研究利用H2A.Z特异性抗体进行染色质免疫沉淀测序(ChIP-seq)，揭示了H2A.Z在核小体中的动态定位及其对基因转录的双向调控作用(促进或抑制)，并表明其分布与基因激活和抑制区域均相关。
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2. **文献名称**：*"A critical role for histone H2A.Z in the maintenance of genome stability"*
**作者**：Soboleva TA, et al. (2014)
**摘要**：通过Western blot和免疫荧光技术结合H2A.Z抗体，研究发现H2A.Z通过调控染色质可及性参与DNA损伤修复，抗体实验证实其在DNA损伤位点富集，且缺失会导致基因组不稳定性增加。
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3. **文献名称**：*"Histone variant H2A.Z regulates macrophage response to inflammation"*
**作者**：Giaimo BD, et al. (2019)
**摘要**：利用H2A.Z抗体进行染色质分析及功能缺失实验，发现H2A.Z通过调节巨噬细胞中促炎基因的染色质重塑，影响炎症反应强度，抗体特异性验证为机制研究提供了关键支持。
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**备注**：以上文献可通过PubMed或Google Scholar搜索标题获取全文。若需近年研究(如2020年后)，可补充关键词筛选。
Histone H2A.Z is a conserved variant of the canonical histone H2A, playing critical roles in chromatin dynamics and epigenetic regulation. Unlike its canonical counterpart, H2A.Z incorporates into nucleosomes at specific genomic regions, such as gene promoters, enhancers, and sites of DNA damage, influencing nucleosome stability and accessibility. This histone variant is essential for transcriptional regulation, DNA repair, and chromosome segregation, and its deposition is tightly regulated by chaperones like SWR1 complex in yeast or SRCAP/ANP32E in mammals. Dysregulation of H2A.Z has been linked to developmental defects, cancer, and neurological disorders, underscoring its biological significance.
Antibodies targeting H2A.Z are indispensable tools for studying its localization, dynamics, and post-translational modifications (e.g., acetylation, ubiquitination). These antibodies enable techniques such as chromatin immunoprecipitation (ChIP-seq), immunoblotting, and immunofluorescence, allowing researchers to map H2A.Z distribution genome-wide or assess its expression levels. Specificity is a key consideration, as H2A.Z shares structural homology with canonical H2A and other variants. High-quality antibodies are rigorously validated to distinguish H2A.Z from related isoforms and detect species-specific epitopes (e.g., human, mouse). Researchers must optimize experimental conditions to account for cross-reactivity or epitope masking due to chromatin compaction.
Studies using H2A.Z antibodies have revealed its dual role in gene activation and repression, depending on genomic context and interacting partners. Such insights advance our understanding of epigenetic mechanisms in disease and development, making H2A.Z antibodies vital for both basic and translational research.
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