首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG即干扰素-γ，是一种多功能细胞因子。它在免疫调节中至关重要，能增强巨噬细胞和NK细胞活性、促进Th1细胞分化。研究机制方面，通过与受体结合激活下游信号通路，调控免疫相关基因表达，在抗感染、抗肿瘤等领域是重要研究靶点。试剂盒检测范围为0.625 pg/mL-40 pg/mL，适用于科研领域研究感染性疾病模型、自身免疫疾病机制、肿瘤免疫微环境分析及药物干预实验中的免疫动态变化监测，为探索Th1型免疫反应、炎症调控及免疫治疗策略提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IfngInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P01581
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of rat IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected402.3612.2242.2932.103201.4301.3761.4031.213100.9160.9090.9130.72350.6170.6260.6220.4322.50.4240.4530.4390.2491.250.3330.3400.3370.1470.6250.2720.2880.2800.09000.1920.1880.190 | ||||||
货期：3-5 working days
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