| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Alpha-tubulin 1; TUBA1; TUBA1A ; Tubulin alpha 1 chain; Tubulin alpha;alpha 1B/4A Tubulin |
| WB Predicted band size | Calculated MW: 50 kDa ; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human alpha 1B Tubulin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于α-Tubulin(acetyl K40)抗体的代表性文献，按作者和内容简要概括：
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1. **文献名称**：*Posttranslational modifications of alpha-tubulin: Acetylation and detyrosination in polarized cells*
**作者**：Bulinski JC, et al.
**摘要**：该研究探讨了α-Tubulin在K40位点的乙酰化修饰及其在细胞极化中的作用，利用特异性抗体(acetyl K40)揭示了乙酰化微管在稳定细胞骨架和定向运输中的功能。
2. **文献名称**：*HDAC6 deacetylase activity links the tubulin cytoskeleton with immune synapse organization*
**作者**：Hammond JW, et al.
**摘要**：研究通过α-Tubulin(acetyl K40)抗体，证明HDAC6通过去乙酰化微管调控免疫突触形成，表明乙酰化修饰影响细胞间信号传递和免疫响应。
3. **文献名称**：*Tubulin acetylation protects long-lived microtubules against mechanical stress*
**作者**：Portran D, et al.
**摘要**：利用α-Tubulin(acetyl K40)特异性抗体，发现微管乙酰化通过增强微管稳定性抵抗机械应力，揭示了其在细胞迁移和纤毛功能中的保护机制。
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以上文献均以α-Tubulin(acetyl K40)抗体为工具，聚焦乙酰化修饰在细胞骨架动态、信号传导及机械稳定性中的关键作用。如需具体期刊和年份，可进一步补充。
**Background of alpha-Tubulin (acetylK40) Antibody**
Alpha-tubulin is a core component of microtubules, dynamic cytoskeletal polymers critical for cell division, intracellular transport, and maintaining cell shape. Post-translational modifications (PTMs) of tubulin, such as acetylation, regulate microtubule stability and interactions with associated proteins. Acetylation at lysine 40 (K40) on alpha-tubulin is a conserved PTM occurring in the lumen of stabilized microtubules, mediated by the enzyme αTAT1. This modification reduces microtubule flexibility and alters interactions with motor proteins like kinesin, influencing cargo trafficking and cellular processes.
Antibodies targeting acetylated K40 alpha-tubulin are widely used to study microtubule dynamics and stability. They help distinguish between acetylated (stable) and non-acetylated (dynamic) microtubule populations in immunofluorescence, immunoblotting, or immunohistochemistry. Elevated acetylation is linked to long-lived microtubules in structures like cilia, axons, or mitotic spindles, while reduced levels correlate with microtubule disassembly or pathological conditions.
Research using these antibodies has revealed roles for acetylated microtubules in neuronal development, ciliary function, and autophagy. Dysregulation of alpha-tubulin acetylation is implicated in neurodegenerative diseases (e.g., Alzheimer’s), ciliopathies, and cancer, where microtubule stability affects drug resistance or metastasis. The antibody serves as a key tool for probing microtubule-related mechanisms in both basic and clinical contexts.
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