首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG 即干扰素 -γ，在免疫调节中发挥关键作用，可增强巨噬细胞活性、促进 Th1 细胞分化、调节 MHC 分子表达等。研究机制上，它通过与受体结合激活下游信号通路，调节众多基因表达，在抗肿瘤、抗感染及自身免疫病中是重要研究靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于多种科研场景，包括但不限于动物免疫机制研究、疫苗效力评估实验、感染性疾病模型中的免疫应答分析以及炎症相关信号通路研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P30123
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 85-105 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 85-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 83-105 | |||||
回收率：
| The recovery of rabbit IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-100 | ||||
| EDTA plasma (n=4) | 95 | 85-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.8622.7132.7882.67320002.2142.2292.2222.10710001.3731.3971.3851.2705000.7550.7310.7430.6282500.4460.4320.4390.3241250.2630.2750.2690.15462.50.2020.1940.1980.08300.1170.1130.115 | ||||||
货期：3-5 working days
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