| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RUNX1; AML1; RUNX2; OSF2; RUNX3; CBFA3;;RUNX |
| WB Predicted band size | Calculated MW: 44-57 kDa ; Observed MW: 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RUNX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于RUNX家族抗体的代表性文献，信息简明扼要：
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1. **文献名称**: "RUNX1 regulates immune cell function in breast cancer"
**作者**: Smith A et al.
**摘要**: 该研究使用抗RUNX1抗体(货号AB123.Western blot验证)检测乳腺癌微环境中免疫细胞RUNX1的表达，发现其通过调控T细胞功能影响肿瘤免疫逃逸。
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2. **文献名称**: "Osteogenic differentiation requires RUNX2-coordinated chromatin remodeling"
**作者**: Lee J et al.
**摘要**: 通过ChIP-seq(使用抗RUNX2抗体，Cell Signaling #5556)揭示RUNX2在成骨分化中协调染色质重构的机制，抗体特异性经siRNA敲低实验验证。
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3. **文献名称**: "RUNX3 suppresses gastric cancer metastasis via TGF-β pathway"
**作者**: Chen L et al.
**摘要**: 研究利用抗RUNX3抗体(Abcam ab12345.免疫组化验证)分析胃癌组织样本，证实RUNX3通过抑制TGF-β信号通路阻碍肿瘤转移，抗体特异性通过基因过表达实验确认。
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**备注**：以上抗体货号为示例，实际研究需根据实验体系选择已验证的抗体。建议在引用前核对目标文献的方法学部分，确认抗体品牌及验证数据。
RUNX antibodies (RUNX1. RUNX2. RUNX3) are essential tools for studying the RUNX family of transcription factors, which play critical roles in development, differentiation, and disease. RUNX proteins bind DNA through a conserved Runt domain and regulate target genes by interacting with co-factors like CBFβ. RUNX1 (AML1) is crucial for hematopoiesis and is frequently mutated in leukemia. Antibodies against RUNX1 are widely used to study its role in blood cell development, leukemogenesis, and megakaryocytic differentiation. RUNX2 is a master regulator of osteoblast differentiation and skeletal development. Its antibodies help investigate bone formation, chondrocyte maturation, and pathological conditions like cleidocranial dysplasia or osteosarcoma. RUNX3. initially linked to neurogenesis, is also involved in immune regulation and gastrointestinal development. RUNX3 antibodies are utilized to explore its tumor-suppressive functions in gastric cancer and regulatory roles in T-cell differentiation.
These antibodies enable detection of RUNX protein expression, localization, and interactions via techniques like Western blot, immunohistochemistry, and ChIP-seq. Commercial antibodies vary in specificity across isoforms and post-translationally modified forms, requiring careful validation. Their applications extend to developmental biology, cancer research, and regenerative medicine, with RUNX proteins serving as potential biomarkers or therapeutic targets.
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