| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | class II beta tubulin isotype; Beta2; Tubulin beta 2 chain; TUBB; TUBB2;;beta 4B/2A/2B Tubulin |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human beta 4B Tubulin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于βIII-Tubulin(TUBB3)抗体的3篇参考文献，可能存在βII与βIII命名的混淆，通常βIII-Tubulin(TUBB3)是神经元标记物：
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1. **文献名称**: *"The distribution of beta III tubulin in neurons of the central nervous system"*
**作者**: Frankfurter A, et al.
**摘要**: 该研究系统描述了βIII-Tubulin(TUBB3)在哺乳动物中枢神经系统神经元中的特异性表达，验证其作为成熟神经元标志物的可靠性，并讨论了其在神经轴突运输中的作用。
2. **文献名称**: *"TUBB3 as a biomarker of chemotherapy resistance in ovarian cancer"*
**作者**: Kavallaris M, et al.
**摘要**: 研究发现βIII-Tubulin在卵巢癌中高表达与紫杉醇类药物耐药性相关，通过抗体检测证实其可作为预测化疗反应的潜在生物标志物。
3. **文献名称**: *"Neuronal differentiation of stem cells tracked by βIII-tubulin antibody labeling"*
**作者**: Fujioka H, et al.
**摘要**: 利用βIII-Tubulin抗体标记，实时追踪胚胎干细胞向神经元分化的动态过程，证明其在神经发育研究中的关键应用价值。
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**注意**：βII-Tubulin(TUBB2)与βIII-Tubulin(TUBB3)属于不同亚型，若需βII特异性文献，建议进一步确认靶点命名及研究背景。
BetaII-tubulin, a specific isoform of the β-tubulin family, is a crucial structural component of microtubules, which play vital roles in cellular processes such as mitosis, intracellular transport, and cell shape maintenance. Encoded by the *TUBB2B* gene in humans, betaII-tubulin is prominently expressed in neurons, where it contributes to axonal growth, neuronal differentiation, and synaptic plasticity. Its expression is not exclusive to neural tissues but is also observed in certain non-neuronal cells during development or regeneration.
Antibodies targeting betaII-tubulin are widely used as markers for neurons and neuronal precursors in immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting. These antibodies help distinguish mature neurons from other cell types, aiding studies in neurodevelopment, neurodegeneration (e.g., Alzheimer’s disease), and neuro-oncology (e.g., glioblastoma). Their specificity for the betaII isoform minimizes cross-reactivity with other β-tubulin isoforms, such as betaIII-tubulin (common in immature neurons) or betaI-tubulin (ubiquitous in most cells).
Commercial betaII-tubulin antibodies are typically raised against conserved epitopes, ensuring reactivity across species like humans, mice, and rats. However, validation in specific experimental contexts (e.g., fixed vs. fresh tissues) is recommended due to potential epitope masking. Overall, betaII-tubulin antibodies remain indispensable tools for exploring neuronal biology and disease mechanisms.
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