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产品描述：人干扰素诱导蛋白35kDa(IFI35)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IFI35含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，本试剂盒适用于体外培养细胞模型、动物实验组织样本以及临床前研究中的体液样本分析，为探索IFI35在免疫调控机制、抗病毒反应研究及药物开发中的生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ21753 ELISA Kit; IFI 35 ELISA Kit; Ifi-35 ELISA Kit; Ifi35 ELISA Kit; IFP 35 ELISA Kit; IFP35 ELISA Kit; IN35 ELISA Kit; IN35_HUMAN ELISA Kit; Interferon induced 35 kDa protein ELISA Kit; Interferon-induced 35 kDa protein ELISA Kit; OTTHUMP00000236626 ELISA Kit; OTTHUMP00000236627 ELISA Kit
缩写：IFI35
Uniprot No.：P80217
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IFI35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 96-102 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 89-100 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 88 | |||||
| Range % | 86-95 | ||||||
回收率：
| The recovery of human IFI35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 99-108 | |||||
| EDTA plasma (n=4) | 93 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.7682.8482.8082.7158001.6221.7091.6661.5734001.0341.1151.0750.9822000.6690.6550.6620.5691000.3230.3460.3350.242500.2270.2220.2250.132250.1440.1340.1390.04600.0960.0900.093 . | |||||||
货期：3-5 working days
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