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Rabbit Monoclonal Phospho-PAK1/2/3(S144+S141+S139) Antibody

  • 中文名： Phospho-PAK1/2/3(S144+S141+S139)抗体
  • 别    名： ADRB2; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; P65-PAK; P68-PAK; PAK1 (phospho S144); PAK2 (phospho S141); PAK3 (phospho S139); ;p-PAK1/2/3 (S144/S141/S154)
货号: IPDX17139
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesADRB2; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; P65-PAK; P68-PAK; PAK1 (phospho S144); PAK2 (phospho S141); PAK3 (phospho S139); ;p-PAK1/2/3 (S144/S141/S154)
WB Predicted band sizeCalculated MW: 58-62 kDa ; Observed MW: 65 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human PAK1 around the phosphorylation site of S144
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于Phospho-PAK1/2/3(S144/S141/S139)抗体的示例参考文献(注：部分内容为模拟示例，实际文献需通过学术数据库核实)：

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1. **文献名称**：*PAK1 phosphorylation at Ser144 activates MAPK signaling to promote breast cancer metastasis*

**作者**：Eswaran, J. et al.

**摘要**：本研究利用Phospho-PAK1(S144)抗体，揭示了PAK1在乳腺癌细胞中通过S144位点磷酸化激活ERK/MAPK通路，促进细胞迁移和侵袭的分子机制。

2. **文献名称**：*Phosphorylation of PAK2 at Ser141 regulates cytoskeletal dynamics in melanoma progression*

**作者**：Arias-Romero, L. et al.

**摘要**：通过Phospho-PAK2(S141)特异性抗体，作者证明黑色素瘤中PAK2的S141磷酸化通过调控Rho GTPase活性驱动细胞骨架重组，增强肿瘤侵袭性。

3. **文献名称**：*PAK3 Ser139 phosphorylation modulates dendritic spine formation in neuronal development*

**作者**：Manser, E. et al.

**摘要**：该研究使用Phospho-PAK3(S139)抗体，发现神经元中PAK3的S139磷酸化通过LIMK1-cofilin通路调控树突棘形态发生，影响突触可塑性。

4. **文献名称**：*Validation of a multiplex assay for detecting PAK1/2/3 activation in clinical tumor samples*

**作者**：Smith, K. et al.

**摘要**：开发了一种基于Phospho-PAK1/2/3(S144/S141/S139)抗体的多重免疫组化方法，用于评估PAK家族激活状态与肿瘤患者预后的相关性。

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**提示**：实际文献需通过PubMed、Web of Science等平台检索关键词“PAK1 Ser144 phosphorylation”“PAK2 Ser141 antibody”等，或参考抗体供应商(如CST、Abcam)官网提供的引用文献列表。

背景信息

The Phospho-PAK1/2/3 (S144+S141+S139) antibody detects endogenous levels of PAK1. PAK2. and PAK3 when phosphorylated at specific regulatory sites: Ser144 (PAK1), Ser141 (PAK2), and Ser139 (PAK3). These p21-activated kinases (PAKs) are critical regulators of cytoskeletal dynamics, cell motility, survival, and proliferation. Their activation is tightly controlled by Rho GTPases (e.g., Cdc42. Rac1), which bind to the autoinhibitory domain, triggering autophosphorylation at these conserved serine residues. This phosphorylation event relieves autoinhibition, enabling kinase activation and downstream signaling. The antibody is particularly useful for studying PAK activation states in cellular pathways, such as MAPK, PI3K/Akt, and Wnt, which are often dysregulated in cancer, neurological disorders, and immune responses. Researchers employ this tool in techniques like Western blotting, immunofluorescence, or immunoprecipitation to explore PAK-related mechanisms in cell migration, invasion, and metastasis. Specificity validation is essential, as cross-reactivity with unrelated phospho-serine motifs may occur. Its application spans cancer research (e.g., breast, glioblastoma), neurodevelopmental studies, and investigations into PAK inhibitors' therapeutic potential. Proper controls, including knockout cells or phosphatase treatment, should confirm phosphorylation-dependent signals.

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