首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠吲哚胺2,3-双加氧酶(IDO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IDO1含量。IDO1即吲哚胺2,3 -双加氧酶1，是一种免疫调节酶。其背景在于它可通过催化色氨酸代谢影响免疫微环境。研究机制方面，它能消耗色氨酸造成T细胞增殖受抑、凋亡增加，还可促进调节性T细胞生成，从而帮助肿瘤逃避机体免疫监视。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可利用开展免疫调控机制研究、药物干预效果评估或疾病模型（如肿瘤、感染性疾病或自身免疫异常）中IDO动态变化的定量分析，为探索IDO相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ido1 ELISA Kit; Ido ELISA Kit; Indo ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; IDO-1 ELISA Kit; EC 1.13.11.52 ELISA Kit; Indoleamine-pyrrole 2,3-dioxygenase ELISA Kit
缩写：IDO1
Uniprot No.：Q9ERD9
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IDO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of rat IDO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 96 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8642.7112.7882.69310001.8521.7241.7881.6935001.0761.0311.0540.9592500.6520.6570.6550.5601250.3610.3770.3690.27462.50.2640.2540.2590.16431.250.1720.1780.1750.08000.0960.0940.095 | ||||||
货期：3-5 working days
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