| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LDHA; LDH-M; LDH1; PIG19; GSD11; TRG5; Proliferation inducing gene 19;;LDHA |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human LDHA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于乳酸脱氢酶(LDH)抗体的3篇参考文献示例(注：文献为示例性质，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *"Targeting lactate dehydrogenase-A inhibits tumorigenesis and tumor progression in mouse models of lung cancer"*
**作者**: Xie et al.
**摘要**: 研究通过特异性LDH-A抗体调控LDH同工酶活性，验证其在肺癌小鼠模型中抑制肿瘤生长和转移的作用，表明LDH-A可能成为癌症代谢治疗的靶点。
2. **文献名称**: *"A monoclonal antibody-based ELISA for detection of human lactate dehydrogenase isoenzyme 1 (LDH-1)"*
**作者**: Smith et al.
**摘要**: 开发了一种基于单克隆抗体的ELISA检测方法，特异性识别LDH-1同工酶，用于心肌梗死和肝损伤的早期临床诊断，具有高灵敏度和特异性。
3. **文献名称**: *"Lactate dehydrogenase B regulates macrophage polarization in tumor microenvironment"*
**作者**: Li et al.
**摘要**: 利用LDH-B抗体研究巨噬细胞代谢重编程，发现LDH-B通过调控乳酸代谢影响肿瘤微环境中巨噬细胞的极化方向，为免疫治疗提供新思路。
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**应用领域扩展**：
- LDH抗体常用于细胞毒性实验(如LDH释放法检测细胞死亡)
- 在神经退行性疾病中，LDH-C4抗体被用于研究线粒体功能障碍机制(如阿尔茨海默病模型)。
建议通过PubMed或Web of Science检索关键词“LDH antibody application”获取最新研究。
Lactate dehydrogenase (LDH) antibodies are immunological tools used to detect and quantify LDH, a critical enzyme involved in cellular energy metabolism. LDH catalyzes the interconversion of pyruvate and lactate during glycolysis, playing a key role in anaerobic respiration. It exists as five tetrameric isoenzymes (LDH-1 to LDH-5), formed by combinations of two subunit types: LDHA (M) and LDHB (H). These isoforms exhibit tissue-specific expression; for example, LDH-1 (H4) is predominant in the heart, while LDH-5 (M4) is enriched in the liver and skeletal muscle.
LDH antibodies are widely employed in research and diagnostics to study enzyme distribution, cellular stress, and disease mechanisms. Elevated LDH levels in blood are nonspecific biomarkers for tissue damage, such as myocardial infarction, hemolysis, or liver disease. In cancer research, LDH-A overexpression is linked to tumor progression, metastasis, and poor prognosis due to its role in the Warburg effect. Antibodies targeting specific LDH isoforms enable precise detection via techniques like Western blotting, immunohistochemistry, or ELISA.
Monoclonal antibodies offer high specificity for individual subunits or isoforms, while polyclonal antibodies may detect broader epitopes. Their applications extend to investigating metabolic adaptations in hypoxia, drug toxicity studies, and monitoring therapeutic responses. Validation of LDH antibodies ensures reliability in distinguishing isoforms, which is crucial for interpreting pathological or physiological conditions tied to metabolic dysregulation.
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