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Rabbit Monoclonal PI3KinaseClass3 Antibody

  • 中文名： PI3KinaseClass3抗体
  • 别    名： PI3-kinase type 3; PI3K type 3; PI3 kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3; Vps 34; Vps34;PI3 kinase p100
货号: IPDX17130
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPI3-kinase type 3; PI3K type 3; PI3 kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3; Vps 34; Vps34;PI3 kinase p100
WB Predicted band sizeCalculated MW: 102 kDa ; Observed MW: 100 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human PI3 kinase p100
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3篇关于Class III PI3K(VPS34)抗体的参考文献，内容精简概括：

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1. **文献名称**: *Regulation of mammalian autophagy by class III phosphatidylinositol 3-kinase*

**作者**: Jaber N. et al.

**摘要**: 该研究利用特异性抗体验证了hVPS34在哺乳动物自噬中的作用，通过siRNA敲低和抗体免疫沉淀证明hVPS34与Beclin-1的相互作用对自噬体形成至关重要。

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2. **文献名称**: *Distinct roles of class III phosphatidylinositol 3-kinase in autophagy and vesicular trafficking*

**作者**: Petiot A. et al.

**摘要**: 通过Western blot和免疫荧光实验，使用针对VPS34的抗体，揭示了其在自噬和溶酶体运输中的双重功能，并验证抗体在多种细胞系中的特异性。

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3. **文献名称**: *Characterization of VPS34-inhibitor complexes and antibody tools for autophagy research*

**作者**: Dowdle W.E. et al.

**摘要**: 研究开发了高特异性抗VPS34单克隆抗体，结合基因敲除细胞系验证其无交叉反应性，并应用于检测VPS34与抑制剂结合的构象变化。

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这些文献均涉及Class III PI3K抗体的实验验证与应用，涵盖自噬机制和抗体工具开发方向。如需具体年份或期刊信息可进一步补充。

背景信息

The PI3K (phosphatidylinositol 3-kinase) Class 3 antibody targets components of the Class III PI3K complex, primarily the catalytic subunit VPS34 (vacuolar protein sorting 34), encoded by the *PIK3C3* gene. Class III PI3K is distinct from Class I and II PI3Ks, as it exclusively generates phosphatidylinositol 3-phosphate (PtdIns3P), a lipid critical for membrane trafficking, autophagy, and lysosomal function. VPS34 forms a core complex with regulatory proteins like Beclin-1 and VPS15. which modulate its activity in autophagy initiation and endosomal sorting. Antibodies against PI3K Class III are essential tools for studying these processes, enabling detection of protein expression, localization, and interaction partners via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Researchers use these antibodies to explore roles in diseases linked to autophagy dysregulation, such as cancer, neurodegenerative disorders, and metabolic conditions. For instance, reduced VPS34 or Beclin-1 levels are associated with impaired autophagy in Alzheimer’s or Parkinson’s disease models. Additionally, these antibodies help investigate crosstalk between autophagy and pathways like mTOR or apoptosis. Specificity validation (e.g., knockout controls) is crucial due to structural similarities among PI3K classes. Overall, PI3K Class III antibodies are pivotal in deciphering cellular homeostasis mechanisms and therapeutic targeting in autophagy-related pathologies.

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