| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Flotillin1 ; Flotillin 1; Reggie 2; FLOT1; Integral membrane component of caveolae; ;Flotillin 1 |
| WB Predicted band size | Calculated MW: 47 kDa ; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Flotillin 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Flotillin1抗体的参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Flotillin-1 regulates oncogenic signaling in neuroblastoma cells*
**作者**：G. Li et al.
**摘要**：该研究利用Flotillin1抗体通过Western blot和免疫共沉淀技术，揭示了Flotillin1在神经母细胞瘤中通过调控EGFR信号通路促进肿瘤细胞增殖和转移的机制。
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2. **文献名称**：*Flotillin-1 facilitates endocytic uptake of influenza virus and modulates viral replication*
**作者**：S. Banerjee et al.
**摘要**：研究通过免疫荧光和流式细胞术(使用Flotillin1特异性抗体)，证明Flotillin1在流感病毒内吞过程中起关键作用，其缺失显著降低病毒进入宿主细胞的效率。
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3. **文献名称**：*Flotillin-1 as a novel biomarker for early detection of Alzheimer's disease*
**作者**：M. R. Hölttä et al.
**摘要**：通过脑脊液样本的ELISA和免疫组化分析(采用高灵敏度Flotillin1抗体)，发现Flotillin1在阿尔茨海默病早期患者中异常聚集，提示其作为潜在生物标志物的价值。
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**备注**：以上文献为示例，实际引用时请核对期刊名称、年份及具体实验细节。如需更多文献，建议在PubMed或Google Scholar中检索关键词“Flotillin1 antibody”+“specific application”(如癌症、病毒、神经疾病等)。
Flotillin-1 (FLOT1) is a conserved, ubiquitously expressed protein belonging to the flotillin family, which includes Flotillin-2 (FLOT2). It is a key component of lipid raft microdomains in cellular membranes, playing critical roles in membrane organization, endocytosis, signal transduction, and cytoskeletal regulation. Flotillin-1 forms hetero-oligomeric complexes with Flotillin-2. contributing to the scaffolding of membrane-associated processes such as receptor tyrosine kinase signaling (e.g., EGFR, insulin receptor) and vesicle trafficking. Its involvement in cellular processes like cell adhesion, membrane protein sorting, and pathogen entry has linked it to various pathologies, including cancer progression, neurodegenerative diseases, and metabolic disorders.
Antibodies targeting Flotillin-1 are essential tools for studying its expression, localization, and function. Commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation, these antibodies help elucidate Flotillin-1’s role in lipid raft dynamics and disease mechanisms. Validation via knockdown models or Flotillin-1-deficient cells is critical to ensure specificity, given its structural similarity to Flotillin-2. Research applications span cancer biology (examining metastasis and drug resistance), neuroscience (exploring synaptic plasticity), and immunology (studying immune cell signaling). Commercial Flotillin-1 antibodies are typically raised in hosts like rabbits or mice, with clones such as EPR6041 being widely utilized. Reliable antibodies enable precise investigation of Flotillin-1’s contributions to cellular homeostasis and pathological states.
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