| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Flotillin1 ; Flotillin 1; Reggie 2; FLOT1; Integral membrane component of caveolae; ;Flotillin 1 |
| WB Predicted band size | Calculated MW: 47 kDa ; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Flotillin 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Flotillin1抗体的参考文献(虚构示例，仅供参考格式)：
1. **"Flotillin-1 regulates EGFR signaling through interaction with lipid rafts"**
*作者：Zhang L, et al.*
*摘要*：研究利用Flotillin1抗体揭示其在细胞膜脂筏中的定位，并发现其通过调控EGFR内吞作用影响肿瘤细胞增殖。
2. **"Antibody-based detection of Flotillin-1 in neurodegenerative disease models"**
*作者：Smith J, et al.*
*摘要*：开发了一种高特异性Flotillin1抗体，用于检测阿尔茨海默病模型中脂筏蛋白聚集现象，发现其与Aβ斑块形成相关。
3. **"Flotillin-1 antibody reveals its role in T-cell receptor signaling"**
*作者：Wang Y, et al.*
*摘要*：通过免疫共沉淀(使用Flotillin1抗体)证明其参与T细胞活化信号通路，并调控免疫突触的形成。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“Flotillin1 antibody”+具体研究领域(如癌症、神经科学等)。
Flotillin-1. encoded by the *FLOT1* gene, is a conserved, ubiquitously expressed protein belonging to the lipid raft-associated flotillin family. It localizes to membrane microdomains (lipid rafts) and intracellular vesicles, playing roles in membrane trafficking, signal transduction, and scaffolding of protein complexes. Structurally, Flotillin-1 contains a prohibitin homology (PHB) domain and forms oligomers, facilitating its interaction with membranes and partner proteins like Flotillin-2. It participates in clathrin-independent endocytosis, cell adhesion, and receptor signaling, and is implicated in pathological processes including cancer metastasis, neurodegeneration, and viral entry.
Flotillin-1 antibodies are critical tools for studying its expression, localization, and function. Commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation, these antibodies help detect Flotillin-1’s molecular weight (~47-50 kDa) and assess its upregulation in cancers (e.g., breast, liver) or dysregulation in neurological disorders (e.g., Alzheimer’s disease). Both polyclonal and monoclonal antibodies are available, with specificity validated via knockout controls. Commercial antibodies often target epitopes in the N-terminal region. Research applications include exploring lipid raft dynamics, endocytic pathways, and Flotillin-1’s role as a biomarker or therapeutic target. Variability in antibody performance across species or experimental conditions necessitates careful validation.
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