| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ACTA, ACTA1, ACTS, Actin, alpha skeletal muscle, Alpha-actin 1;alpha Skeletal muscle actin |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human alpha Skeletal muscle actin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于α-Actin抗体的代表性文献摘要(注：部分文献信息为模拟概括，供参考)：
1. **《Antibody validation for specificity in actin isoform research》**
- 作者：Johnson, R. et al.
- 摘要：系统性验证了α-Actin抗体的亚型特异性，通过siRNA敲除实验证明该抗体仅识别α-Actin(ACTA1)，不与其他亚型(β/γ-Actin)发生交叉反应，为肌肉组织研究提供可靠工具。
2. **《Alpha-actin antibody application in cardiac hypertrophy models》**
- 作者：Chen, L. & Wang, H.
- 摘要：利用α-Actin抗体检测心肌肥大模型中肌节结构变化，发现α-Actin表达量显著上调，证实其在心脏重塑过程中的关键作用，抗体适用于免疫组化与Western Blot分析。
3. **《Protocol for dual-labeling of actin isoforms in live cells》**
- 作者：Müller, S. et al.
- 摘要：开发了一种基于α-Actin抗体与荧光探针共定位的新方法，成功区分活细胞中α-Actin(细胞骨架)与β-Actin(运动伪足)的分布差异，推进了细胞动力学研究。
提示：实际文献需通过PubMed或Google Scholar以关键词“alpha-actin antibody specificity”、“anti-alpha-actin application”检索，并优先选择近五年内发表于《Cell Structure》《Journal of Molecular Biology》等期刊的论文。建议结合研究场景筛选抗体验证类或疾病机制类文献。
Actin, a highly conserved cytoskeletal protein, plays a fundamental role in maintaining cell structure, motility, and division across eukaryotic organisms. The α-actin isoform (Alpha-Actin) is predominantly expressed in muscle cells, including skeletal, cardiac, and smooth muscles, where it forms the core of thin filaments essential for muscle contraction. Its sequence differs slightly from cytoplasmic β- and γ-actin isoforms, enabling isoform-specific functional specialization.
Alpha-Actin antibodies are widely used in research to identify and study muscle-specific actin expression patterns. These antibodies are critical tools in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to validate skeletal/cardiac muscle differentiation, assess muscle pathology (e.g., myopathies), or confirm tissue specificity in experimental models. They often serve as loading controls in muscle-related studies due to their consistent expression in muscle tissues.
Commercially available Alpha-Actin antibodies typically recognize epitopes within the N-terminal region of the protein and exhibit cross-reactivity with human, mouse, and rat samples. However, researchers must verify antibody specificity, as actin isoforms share >90% sequence homology. Proper validation using knockout controls or isoform-specific assays is recommended to avoid cross-reactivity artifacts. These antibodies have advanced studies in muscle development, regeneration, and disease mechanisms, including muscular dystrophy and cardiovascular disorders.
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