| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p110Beta; PI3K; PI3K beta; PI3KCB; PIK3C1; Pik3cb;;PI3 kinase p110 beta |
| WB Predicted band size | Calculated MW: 123 kDa ; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PI3 kinase p110 beta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PI3K p110β抗体的3篇参考文献，按研究内容与抗体应用方向整理：
1. **文献名称**：*"Essential role of the p110β subunit of phosphoinositide 3-OH kinase in male fertility"*
**作者**：Ciraolo E. et al.
**摘要**：该研究利用p110β特异性抗体，通过基因敲除小鼠模型揭示p110β亚型在精子形成中的关键作用，并证实其与p85调节亚基的相互作用机制。
2. **文献名称**：*"The p110β isoform of phosphoinositide 3-kinase signals downstream of G protein-coupled receptors and is functionally redundant with p110γ"*
**作者**：Guillermet-Guibert J. et al.
**摘要**：文章通过免疫沉淀和Western blot技术(使用p110β特异性抗体)，证明p110β在G蛋白偶联受体(GPCR)信号传导中的独特功能，并发现其与p110γ的部分功能重叠。
3. **文献名称**：*"PTEN-deficient cancers depend on PIK3CB"*
**作者**：Jia S. et al.
**摘要**：该研究利用p110β抗体验证蛋白表达，结合基因沉默实验，发现PTEN缺失的肿瘤细胞高度依赖p110β(而非p110α)，为靶向治疗提供了理论依据。
**备注**：上述文献中抗体主要用于检测p110β的表达或与其他蛋白的相互作用，相关研究多聚焦于其在癌症、信号通路及生殖生物学中的功能。建议通过PubMed或Web of Science以“PI3K p110β antibody”及作者名进一步检索全文。
The PI3K p110β antibody is a crucial tool for studying the class IA phosphatidylinositol 3-kinase (PI3K) isoform p110β, encoded by the *PIK3CB* gene. PI3Ks regulate cell growth, survival, and metabolism by generating lipid secondary messengers. Unlike the ubiquitously expressed p110α isoform, p110β plays distinct roles in signaling, particularly in PTEN-deficient cancers where it drives tumor progression independently of upstream receptor tyrosine kinase activation. Its unique ability to interact with G-protein-coupled receptors (GPCRs) and small GTPases further underscores its functional specialization.
Antibodies targeting p110β are widely used to assess its expression, subcellular localization, and activation status in cancer research, drug development, and mechanistic studies. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity is critical, as p110β shares structural homology with other PI3K isoforms (e.g., p110α), necessitating rigorous validation to avoid cross-reactivity.
Research using these antibodies has highlighted p110β's therapeutic relevance, particularly in cancers resistant to p110α-targeted therapies. Inhibitors selective for p110β are under investigation, emphasizing the antibody's role in biomarker analysis and preclinical validation. Additionally, studies leveraging p110β antibodies have elucidated its non-catalytic functions in cell migration and cytoskeletal remodeling.
Overall, p110β antibodies are indispensable for dissecting PI3K signaling complexity and advancing targeted cancer therapies.
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