| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hMLKL ; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase;;p-Mlkl (S345) |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | A synthesized peptide derived from mouse Mlkl around the phosphorylation site of S345 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-MLKL(S345)抗体的三篇参考文献，涵盖其作用机制、结构研究及疾病应用：
1. **"Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase"**
*作者：Sun, L. et al. (Cell, 2012)*
**摘要**：该研究首次确定MLKL是RIP3激酶的下游效应分子，介导坏死性凋亡。通过Phospho-MLKL(S345)抗体，作者验证了RIP3介导的MLKL磷酸化是坏死性凋亡的关键步骤，并证明其导致细胞膜破裂。
2. **"Activation of the pseudokinase MLKL unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death"**
*作者：Hildebrand, J. M. et al. (PNAS, 2014)*
**摘要**：研究揭示了MLKL的激活机制，发现S345磷酸化触发其构象变化，释放四螺旋束结构域并定位至细胞膜。文中使用Phospho-MLKL(S345)抗体证实了磷酸化与细胞死亡的相关性。
3. **"RIPK3 activates parallel pathways of MLKL-driven necroptosis and FADD-mediated apoptosis"**
*作者：Kaiser, W. J. et al. (Cell Death & Differentiation, 2014)*
**摘要**：研究探讨了RIPK3通过磷酸化MLKL(S345)诱导坏死性凋亡的机制，并对比其与凋亡通路的交叉对话。通过特异性抗体，作者在多种细胞模型中观察到磷酸化MLKL的积累。
这些文献分别从分子机制、结构功能及通路交叉角度阐明了Phospho-MLKL(S345)的作用，并验证了相关抗体的应用。
The Phospho-MLKL(S345) antibody is a critical tool for studying necroptosis, a programmed form of inflammatory cell death. MLKL (Mixed Lineage Kinase Domain-Like) is the terminal executor of necroptosis, activated via phosphorylation by RIPK3 (Receptor-Interacting Protein Kinase 3) in response to stimuli like TNFα, viral infections, or caspase inhibition. Phosphorylation at serine 345 (human) or the analogous serine 346 (mouse) induces MLKL oligomerization and translocation to the plasma membrane, disrupting membrane integrity and causing cell death. This phosphorylation event serves as a key biomarker for necroptosis activation.
The Phospho-MLKL(S345) antibody specifically detects this post-translational modification, enabling researchers to confirm MLKL activation in experimental models. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study necroptosis in contexts such as inflammatory diseases, neurodegenerative disorders, cancer, and pathogen-host interactions. Validated for use in human, mouse, and rat samples, this antibody helps delineate necroptosis pathways and evaluate therapeutic agents targeting RIPK1/RIPK3/MLKL signaling. Its specificity for the phosphorylated form ensures accurate detection of pathway activation, distinguishing necroptosis from apoptosis or accidental necrosis.
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