首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠4-羟基壬烯酸(HNE)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的HNE含量。HNE即人中性粒细胞弹性蛋白酶，在人体免疫防御中起重要作用。但过度激活会引发多种疾病。研究其机制可聚焦在它对底物蛋白的降解、信号通路的调控等方面，以此为靶点有望开发出治疗相关病症如肺部炎症疾病的药物。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可应用于氧化应激相关机制研究、抗氧化药物干预效果评估，或心血管疾病、代谢综合征等动物模型中脂质过氧化水平的动态监测，为科研人员提供灵敏、可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HNE
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 83-92 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 89-99 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of rat HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-95 | ||||
| EDTA plasma (n=4) | 98 | 94-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.1180.1100.114 5000.1840.1920.188 2500.2650.2730.269 1250.4740.4660.470 62.50.6250.6150.620 31.21.1291.0741.102 15.62.1822.2812.232 02.8772.9852.931 | ||||||
货期：3-5 working days
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